Lipidomic signatures from physically frail and robust older adults at hospital admission Ramírez Vélez, Robinson Correa Rodríguez, María Frailty Lipidomic Ceramides Cholesterol Phosphatidylcholines Biomarker Older adults Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was funded by a Gobierno de Navarra project Resolucion grant 2186/2014 and acknowledged with the "Beca Ortiz de Landazuri" as the best research clinical project in 2014, as well as by a research grant PI17/01814 of the Ministerio de Economia, Industria y Competitividad (ISCIII, FEDER). A.G.-H. is a Miguel Servet Fellow (Instituto de Salud Carlos III -CP18/0150). N.M.-V. received funding from "la Caixa" Foundation (ID 100010434), under agreement LCF/PR/PR15/51100006. R.R.-V. is funded in part by a Postdoctoral Fellowship Resolution ID 420/2019 of the Universidad Publica de Navarra. Identifying serum biomarkers that can predict physical frailty in older adults would have tremendous clinical value for primary care, as this condition is inherently related to poor quality of life and premature mortality. We compared the serum lipid profile of physically frail and robust older adults to identify specific lipid biomarkers that could be used to assess physical frailty in older patients at hospital admission. Fortythree older adults (58.1% male), mean (range) age 86.4 (78–100 years) years, were classified as physically frail (n = 18) or robust (n = 25) based on scores from the Short Physical Performance Battery (≤ 6 points). Nontargeted metabolomic study by ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) analysis with later bioinformatics data analysis. Once the significantly different metabolites were identified, the KEGG database was used on them to establish which were the metabolic pathways mainly involved. Area under receiver-operating curve (AUROC) analysis was used to test the discriminatory ability of lipid biomarkers for frailty based on the Short Physical Performance Battery. We identified a panel of five metabolites including ceramides Cer (40:2), Cer (d18:1/20:0), Cer (d18:1/23:0), cholesterol, and phosphatidylcholine (PC) (14:0/20:4) that were significantly increased in physically frail older adults compared with robust older adults at hospital admission. The most interesting in the physically frail metabolome study found with the KEGG database were the metabolic pathways, vitamin digestion and absorption, AGERAGE signaling pathway in diabetic complications, and insulin resistance. In addition, Cer (40:2) (AUROC 0.747), Cer (d18:1/23:0) (AUROC 0.720), and cholesterol (AUROC 0.784) were identified as higher values of physically frail at hospital admission. The non-targeted metabolomic study can open a wide view of the physically frail features changes at the plasma level, which would be linked to the physical frailty phenotype at hospital admission. Also, we propose that metabolome analysis will have a suitable niche in personalized medicine for physically frail older adults. 2022-02-24T08:31:01Z 2022-02-24T08:31:01Z 2022-02-04 journal article Ramírez-Vélez, R... [et al.]. Lipidomic signatures from physically frail and robust older adults at hospital admission. GeroScience (2022). [https://doi.org/10.1007/s11357-021-00511-1] http://hdl.handle.net/10481/72984 10.1007/s11357-021-00511-1 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Springer