Clay Mineral Minerals as a Strategy for Biomolecule Incorporation: Amino Acids Approach Brandão Lima, Luciano C. Viseras Iborra, César Antonio Sepiolite Montmorillonite Adsorption L-lysine L-methionine L-tryptophan Nutrition Ruminants The authors thank CAPES, CNPq, and UFPI for financial and/or structural support. The potential use of amino acids by ruminal microorganisms converting them into microbial protein for ruminants makes it challenging to supplement these nutrients in an accessible form in animals’ diets. Several strategies to protect amino acids from ruminal degradation were reported, producing amino acids available for the protein used in the intestine called “bypass.” The intercalation of biomolecules in clay mineral minerals has gained notoriety due to its ability to support, protect, transport, physicochemical properties and non-toxicity. This study aimed to investigate the incorporation of L-lysine (Lys), L-methionine (Met), and L-tryptophan (Trp) amino acids in the clay minerals sepiolite (Sep) and Veegum® (Veg) using the adsorption method. The characterization techniques of X-ray diffraction and infrared spectroscopy indicated the presence of biomolecules in the inorganic matrices. Elemental and thermal analyzes monitored the percentages of incorporated amino acids. They showed better incorporation capacities for Veg, such as Met-Veg < Lys-Veg < Trp-Veg and Lys-Sep < Met-Sep < Trp-Sep for sepiolite, except for the incorporation of Met. Matrices provide a promising alternative for planning the administration of biomolecules, using essential amino acids as models, and may offer an alternative to improve functional diet strategies. 2022-02-24T07:30:28Z 2022-02-24T07:30:28Z 2021-12-22 journal article Brandão-Lima, L.C... [et al.]. Clay Mineral Minerals as a Strategy for Biomolecule Incorporation: Amino Acids Approach. Materials 2022, 15, 64. [https://doi.org/10.3390/ma15010064] http://hdl.handle.net/10481/72981 10.3390/ma15010064 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI