Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos Yokoshi, Moe Cambón Gandarias, Manuel Grant-in-Aid for Transformative Research Areas (A) (Research in a ProposedResearch Area) [21H05742]; Grant-inAid for Scientific Research on Innovative Areas (Research in a Proposed Research Area) [20H05357]; Grant-in-Aid for Scientific Research (B) [19H03154]; Grant-in-Aid for Challenging Research (Exploratory) [19K22378]; Grant-inAid for Research Activity Start-up [18H06040] from the Japan Society for the Promotion of Science; Grant-in-Aid for Leading Initiative for ExcellentYoungResearchers from the Ministry of Education, Culture, Sports, Science and Technology in Japan; Tomizawa Jun-ichi & Keiko Fund of Molecular Biology Society of Japan for Young Scientist; research grants from the Mochida Memorial Foundation for Medical and Pharmaceutical Research; Nakajima Foundation; Inamori Foundation; Takeda Science Foundation; Sumitomo Foundation; Senri Life Science Foundation; Mitsubishi Foundation; M.Y. is supported by the Grant-in-Aid for Early-Career Scientists [20K15710] from the Japan Society for the Promotion of Science; Employment Stability Support for Young Researchers from the University of Tokyo; JST, ACT-X [JPMJAX211J]; K.K. is supported by the Grant-in-Aid for Research Activity Start-up [21K20627] from the Japan Society for the Promotion of Science; M.C. is supported by the FPI research grant [FPI2015/074837] from the MINECO-Feder; FisyMat PhD Student Research program from University of Granada. Funding for open access charge: Japan Society for the Promotion of Science. Regulatory interactions between enhancers and core promoters are fundamental for the temporal and spatial specificity of gene expression in development. The central role of core promoters is to initiate productive transcription in response to enhancer’s activation cues. However, it has not been systematically assessed how individual core promoter elements affect the induction of transcriptional bursting by enhancers. Here, we provide evidence that each core promoter element differentially modulates functional parameters of transcriptional bursting in developing Drosophila embryos. Quantitative live imaging analysis revealed that the timing and the continuity of burst induction are common regulatory steps on which core promoter elements impact. We further show that the upstream TATA also affects the burst amplitude. On the other hand, Inr, MTE and DPE mainly contribute to the regulation of the burst frequency. Genome editing analysis of the pair-rule gene fushi tarazu revealed that the endogenous TATA and DPE are both essential for its correct expression and function during the establishment of body segments in early embryos. We suggest that core promoter elements serve as a key regulatory module in converting enhancer activity into transcription dynamics during animal development. 2022-02-23T09:33:50Z 2022-02-23T09:33:50Z 2021-12-13 info:eu-repo/semantics/article Moe Yokoshi... [et al.]. Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos, Nucleic Acids Research, Volume 50, Issue 1, 11 January 2022, Pages 92–107, [https://doi.org/10.1093/nar/gkab1177] http://hdl.handle.net/10481/72957 10.1093/nar/gkab1177 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Oxford University Press