Citrate Stabilizes Hydroxylapatite Precursors: Implications for Bone Mineralization Ruiz Agudo, Encarnación Di Lorenzo, Fulvio Ibáñez Velasco, Aurelia María Rodríguez Navarro, Carlos Manuel Citrate Calcium phosphate Liquid-like precursor Prenucleation species Amorphous calcium phosphate This research was funded by the Spanish Government (grant nos. RTI2018.099565.B.I00 and CGL2015-64683-P), the European Commission (ERDF funds), the University of Granada ("Unidad Cientifica de Excelencia" UCE-PP201605), and the Junta de Andalucia (no. P11-RNM-7550 and research group RNM-179). The authors thank M. Abad and Haidour Benamin from CIC-UGR for their assistance during microscopy and NMR studies. C.R.A. thanks project A7 from SFB1214 (DFG-Deutsche Forschungsgemeinschaf) and Zukunftstkolleg (University of Konstanz). Mineralization of hydroxylapatite (HAp), the main inorganic phase in bone, follows nonclassical crystallization routes involving metastable precursors and is strongly influenced by organic macromolecules. However, the effect of small organic molecules such as citrate on the formation of HAp is not well constrained. Using potentiometric titration experiments and titration calorimetry, in combination with a multianalytical approach, we show that citrate stabilizes prenucleation species as well as a liquid-like calcium phosphate precursor formed before any solid phase nucleates in the system. The stabilization of a liquid-like precursor phase could facilitate infiltration into the cavities of the collagen fibrils during bone mineralization, explaining the enhancement of collagen-mediated mineralization by citrate reported in previous studies. Hence, citrate can influence bone mineralization way before any solid phase (amorphous or crystalline) is formed. We also show that HAp formation after amorphous calcium phosphate (ACP) in the absence and presence of citrate results in nanoplates of about 5−12 nm thick, elongated along the c axis. Such nanoplates are made up of HAp nanocrystallites with a preferred c axis orientation and with interspersed ACP. The nanoplatelet morphology, size, and preferred crystallographic orientation, remarkably similar to those of bone HAp nanocrystals, appear to be an intrinsic feature of HAp formed from an amorphous precursor. Our results challenge current models for HAp mineralization in bone and the role of citrate, offering new clues to help answer the long-standing question as to why natural evolution favored HAp as the mineral phase in bone. 2022-02-11T07:39:59Z 2022-02-11T07:39:59Z 2021-05-11 info:eu-repo/semantics/article ACS Biomater. Sci. Eng. 2021, 7, 2346−2357. [https://doi.org/10.1021/acsbiomaterials.1c00196] http://hdl.handle.net/10481/72791 10.1021/acsbiomaterials.1c00196 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España American Chemical Society