<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/72523">
      <dc:title>Circulating Tumor Cells Enumeration from the Portal Vein for Risk Stratification in Early Pancreatic Cancer Patients</dc:title>
      <dc:creator>Padillo Ruiz, Javier</dc:creator>
      <dc:creator>Serrano Fernández, María José</dc:creator>
      <dc:subject>Circulating tumor cell</dc:subject>
      <dc:subject>Cluster</dc:subject>
      <dc:subject>Portal vein</dc:subject>
      <dc:subject>Central venous catheter</dc:subject>
      <dc:subject>Pancreatic cancer</dc:subject>
      <dc:subject>Early stage</dc:subject>
      <dc:subject>Death risk stratification</dc:subject>
      <dc:description>This research was funded by Carlos III Health Institute (Health Research Fund) grant number PI16/01465 and PI19/01821 (Co-financed by the European Regional Development Fund "A way to make Europe").</dc:description>
      <dc:description>Background. Effective biomarkers are needed to enable personalized medicine for pancreatic&#xd;
cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single&#xd;
circulating tumor cell (CTC) and CTC clusters from the central venous catheter (CVC) and portal&#xd;
blood (PV). Methods. In total, 7 mL of PV and CVC blood from 35 patients with early pancreatic&#xd;
cancer were analyzed. CTC were isolated using a positive immunomagnetic selection. The detection&#xd;
and identification of CTC were performed by immunocytochemistry (ICC) and were analyzed by&#xd;
Epi-fluorescence and confocal microscopy. Results. CTC and the clusters were detected both in PV&#xd;
and CVC. In both samples, the CTC number per cluster was higher in patients with grade three&#xd;
or poorly differentiated tumors (G3) than in patients with well (G1) or moderately (G2) differentiated.&#xd;
Patients with fewer than 185 CTC in PV exhibited a longer OS than patients with more than&#xd;
185 CTC (24.5 vs. 10.0 months; p = 0.018). Similarly, patients with fewer than 15 clusters in PV showed&#xd;
a longer OS than patients with more than 15 clusters (19 vs. 10 months; p = 0.004). These significant&#xd;
correlations were not observed in CVC analyses. Conclusions. CTC presence in PV could be an&#xd;
important prognostic factor to predict poor prognosis in early pancreatic cancer. In addition, the&#xd;
number of clustered-CTC correlate to a tumor negative differentiation degree and, therefore, could&#xd;
be used as a diagnostic biomarker for pancreatic cancer.</dc:description>
      <dc:date>2022-01-27T12:17:11Z</dc:date>
      <dc:date>2022-01-27T12:17:11Z</dc:date>
      <dc:date>2021-12-07</dc:date>
      <dc:type>info:eu-repo/semantics/article</dc:type>
      <dc:identifier>Padillo-Ruiz, J... [et al.]. Circulating Tumor Cells Enumeration from the Portal Vein for Risk Stratification in Early Pancreatic Cancer Patients. Cancers 2021, 13, 6153. [https://doi.org/10.3390/cancers13246153]</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/72523</dc:identifier>
      <dc:identifier>10.3390/cancers13246153</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>MDPI</dc:publisher>
   </ow:Publication>
</rdf:RDF>