A FRET pair for quantitative and superresolution imaging of amyloid fibril formation Ruiz-Arias, Álvaro Jurado Palomares, Rocío Fueyo González, Francisco Herranz, Rosario Gálvez Rodríguez, Natividad González Vera, Juan Antonio Orte Gutiérrez, Ángel Amyloid fibrils Protein aggregation Solvatochromic dyes Biomarkers FLIM STED microscopy This work was supported by grant CTQ2017–85658-R funded by MCIN/AEI/10.13039/501100011033/ FEDER “Una manera de hacer Europa”, and grants PID2019–104366RB-C22 and PID2020–114256RB-I00 funded by MCIN/AEI/10.13039/501100011033 . Funding for open access charge: Universidad de Granada / CBUA. We acknowledge the Centro de Instrumentación Científica (CIC) of Universidad de Granada for use of the TEM facilities. Á.R.-A. thanks the Spanish Ministerio de Educación y Formación Profesional for a FPU Ph.D. studentship. The presence of neuritic plaques and amyloid fibrils arising from the misfolding of certain proteins is the principal molecular indicator of neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Methodologies for studying the early stages of amyloid aggregation are rapidly arising to provide a better understanding of the mechanism of fibrillization and cytotoxicity and to identify potential targets for diagnosis and therapy. The method presented here involves the simultaneous use of two different fluorophores, a quinolimide derivative and Nile Blue A. These are capable of interacting with and reporting on the formation of preamyloid aggregates and fibrils of apoferritin through fluorescence resonance energy transfer (FRET), which occurs between them, thus maximizing the contrast in detection and quantitative information of such amyloid species by using multidimensional fluorescence lifetime imaging microscopy (FLIM). 2021-11-24T11:59:22Z 2021-11-24T11:59:22Z 2022-01-01 info:eu-repo/semantics/article Ruiz-Arias, Á., Jurado, R., Fueyo-González, F., Herranz, R., Gálvez, N., González-Vera, J. A., & Orte, A. (2022). A FRET pair for quantitative and superresolution imaging of amyloid fibril formation. Sensors and Actuators B: Chemical, 350, 130882. [https://doi.org/10.1016/j.snb.2021.130882] http://hdl.handle.net/10481/71723 10.1016/j.snb.2021.130882 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España Elsevier