The role of borosilicate glass in Miller–Urey experiment Criado Reyes, Joaquín García Ruiz, Juan Manuel The authors thank the European Research Council under the European Union's seventh Framework Program (FP7/2007-2013)/ERC grant agreement no. 340863 and the Spanish "Ministerio de Educacion y Ciencia" for the financial support of the project CGL2016-78971-P, the Italian Space Agency for co-funding the Life in Space project (ASI N. 2019-3-U.0) and MIUR 2017-PNR cod. 2017BMK8JR. This work is supported by the Italian Space Agency (ASI) DC-VUM-2017-034 contratto ASI N. 2019-3-U.0, CUP F86C16000000006 "Vita nello spazioOrigine, presenza, persistenza della vita nello spazio, dalle molecole agli estremofili". We have designed a set of experiments to test the role of borosilicate reactor on the yielding of the Miller–Urey type of experiment. Two experiments were performed in borosilicate flasks, two in a Teflon flask and the third couple in a Teflon flask with pieces of borosilicate submerged in the water. The experiments were performed in CH4, N2, and NH3 atmosphere either buffered at pH 8.7 with NH4Cl or unbuffered solutions at pH ca. 11, at room temperature. The Gas Chromatography-Mass Spectroscopy results show important differences in the yields, the number of products, and molecular weight. In particular, a dipeptide, multi-carbon dicarboxylic acids, PAHs, and a complete panel of biological nucleobases form more efficiently or exclusively in the borosilicate vessel. Our results offer a better explanation of the famous Miller’s experiment showing the efficiency of borosilicate in a triphasic system including water and the reduced Miller–Urey atmosphere. 2021-11-18T12:31:11Z 2021-11-18T12:31:11Z 2021-10-25 journal article Criado-Reyes, J... [et al.]. The role of borosilicate glass in Miller–Urey experiment. Sci Rep 11, 21009 (2021). [https://doi.org/10.1038/s41598-021-00235-4] http://hdl.handle.net/10481/71602 10.1038/s41598-021-00235-4 eng info:eu-repo/grantAgreement/EC/FP7/340863 http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Nature