Antioxidant and antiproliferative potential of ethanolic extracts from Moringa oleifera, Tropaeolum tuberosum and Annona cherimola in colorrectal cancer cells Fuel, Marco Mesas Hernández, Cristina Martínez Martínez, Rosario Ortiz Quesada, Raúl Quiñonero Muñoz, Francisco José Prados Salazar, José Carlos Porres Foulquie, Jesús María Melguizo Alonso, Consolación Moringa oleifera Tropaeolum tuberosum Annona cherimola Ethanolic extract Colon cancer 5-fluorouracil This research was funded by the CTS-107 and AGR145 Groups from the Granada University. M. Fuel obtained all plants of the study from Ecuador. A part of this study has been funded by the 2018 Research Initiation Grants Program for Master Students of the Vice-Rectorate for Research and Knowledge Transfer of the University of Granada. Moringa oleifera, Tropaeolum tuberosum and Annona cherimola are medicinal plants traditionally used in Ecuador. However, their therapeutic properties are not completely known. We analyzed chromatographically ethanolic extracts of the seeds of M. oleifera, A. cherimola and the tubers of T. tuberosum; all presented a high content of polyphenols. The extract of A. cherimola showed the highest antioxidant activity and M. oleifera had the highest capacity to enhance the activity of detoxifying enzymes such as glutathione S-transferase and quinone oxidoreductase. The antitumor effect of these extracts was evaluated in vitro with colorectal cancer (CRC) cell lines T84, HCT-15, SW480 and HT-29, as well as with cancer stem cells (CSCs). A. cherimola and M. oleifera extracts presented the lowest IC50 in T-84 and HCT-15 (resistant) cells, respectively, as well as the highest level of inhibition of proliferation in multicellular tumor spheroids of HCT-15 cells. The inhibitory effect on CSCs is noteworthy because in vivo, these cells are often responsible for cancer recurrences and resistance to chemotherapy. Moreover, all extracts showed a synergistic activity with 5-Fu. The antiproliferative mechanism of the extracts was related to overexpression of caspases 9, 8 and 3 and increased production of reactive oxygen species. In addition, we observed cell death by autophagy in M. oleifera and T. tuberosum extracts. Therefore, these ethanolic extracts are excellent candidates for future molecular analysis of the presence of bioactive compounds and in vivo studies which could improve colon cancer therapy. 2021-11-03T08:55:32Z 2021-11-03T08:55:32Z 2021-09-30 info:eu-repo/semantics/article Marco Fuel... [et al.]. Antioxidant and antiproliferative potential of ethanolic extracts from Moringa oleifera, Tropaeolum tuberosum and Annona cherimola in colorrectal cancer cells, Biomedicine & Pharmacotherapy, Volume 143, 2021, 112248, ISSN 0753-3322, [https://doi.org/10.1016/j.biopha.2021.112248] http://hdl.handle.net/10481/71246 10.1016/j.biopha.2021.112248 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Elsevier