Placental DNA methylation signatures of maternal smoking during pregnancy and potential impacts on fetal growth Lacasaña Navarro, Marina González Alzaga, Beatriz Fernández Cabrera, Mariana Fátima Carmona Sáez, Pedro Martorell Marugán, Jordi Fernández Cabrera, Mariana Fátima Gómez-Martín, Antonio We would like to thank all the families that participated in these studies for their generous contribution. Detailed acknowledgements and funding can be found in Sup plementary Material. Supplementary information The online version contains supplementary material available at https://doi.org/10.1038/s41467-021-24558-y Maternal smoking during pregnancy contributes to poor birth outcomes. Here the authors perform a meta-analysis of the associations between maternal smoking during pregnancy and placental DNA methylation and identify links between these and poor birth outcomes, which may better inform the mechanisms through which smoking impacts placental function and fetal growth. Maternal smoking during pregnancy (MSDP) contributes to poor birth outcomes, in part through disrupted placental functions, which may be reflected in the placental epigenome. Here we present a meta-analysis of the associations between MSDP and placental DNA methylation (DNAm) and between DNAm and birth outcomes within the Pregnancy And Childhood Epigenetics (PACE) consortium (N = 1700, 344 with MSDP). We identify 443 CpGs that are associated with MSDP, of which 142 associated with birth outcomes, 40 associated with gene expression, and 13 CpGs are associated with all three. Only two CpGs have consistent associations from a prior meta-analysis of cord blood DNAm, demonstrating substantial tissue-specific responses to MSDP. The placental MSDP-associated CpGs are enriched for environmental response genes, growth-factor signaling, and inflammation, which play important roles in placental function. We demonstrate links between placental DNAm, MSDP and poor birth outcomes, which may better inform the mechanisms through which MSDP impacts placental function and fetal growth. 2021-10-20T08:07:55Z 2021-10-20T08:07:55Z 2021-08-24 info:eu-repo/semantics/article Everson, T.M., Vives-Usano, M., Seyve, E. et al. Placental DNA methylation signatures of maternal smoking during pregnancy and potential impacts on fetal growth. Nat Commun 12, 5095 (2021). [https://doi.org/10.1038/s41467-021-24558-y] http://hdl.handle.net/10481/71000 10.1038/s41467-021-24558-y eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Nature Research