Extraction of the antioxidant phytocomplex from wine-making by-products and sustainable loading in phospholipid vesicles specifically tailored for skin protection Perra, Matteo Lozano Sánchez, Jesús Leyva Jiménez, Francisco Javier Segura Carretero, Antonio Skin delivery Grape pomace Ethanol-water extraction Phospholipid vesicles Fibroblasts Antioxidant The authors thank for technical and human support provided by TEM measurements carried out in the SGIker Polymer Characterization (UPV/EHU/ERDF, EU) . The authors also thank: MIUR and PON R&I for financing the PhD grant; BESTMEDGRAPE - ENI CBC Med project for providing analytical equipment and materials; Cantina Santadi for providing Carignano grape pomace. The authors acknowledge the CeSAR (Centro Servizi d'Ateneo per la Ricerca) of the University of Cagliari, Italy for the granulometry experiments performed with Mal-vern Mastersizer 3000. The present study is aimed at valorizing grape pomace, one of the most abundant winery-making by-products of the Mediterranean area, through the extraction of the main bioactive compounds from the skin of grape pomace and using them to manufacture innovative nanoformulations capable of both avoiding skin damages and promoting skincare. The phytochemicals were recovered through maceration in hydroethanolic solution. Catechin, quercetin, fisetin and gallic acid, which are known for their antioxidant power, were detected as the main compounds of the extract. Liposomes and phospholipid vesicles modified with glycerol or Montanov 82 (R) or a combination of both, were used as carriers for the extract. The vesicles were small (-183 nm), slightly polydispersed (PI >= 0.28), and highly negatively charged (--50 mV). The extract was loaded in high amounts in all vesicles (-100%) irrespective of their composition. The antioxidant activity of the extract, measured by using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, was 84 +/- 1%, and slightly increased when loaded into the vesicles (-89%, P < 0.05). The grape pomace extract loaded vesicles were highly biocompatible and able to protect fibroblasts (3T3) from the oxidative stress induced by hydrogen peroxide. 2021-10-19T12:22:18Z 2021-10-19T12:22:18Z 2021-07-29 info:eu-repo/semantics/article Matteo Perra... [et al.]. Extraction of the antioxidant phytocomplex from wine-making by-products and sustainable loading in phospholipid vesicles specifically tailored for skin protection, Biomedicine & Pharmacotherapy, Volume 142, 2021, 111959, ISSN 0753-3322, [https://doi.org/10.1016/j.biopha.2021.111959] http://hdl.handle.net/10481/70978 10.1016/j.biopha.2021.111959 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España Elsevier