Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis Diaz, Caridad Jiménez Luna, Cristina Diéguez Castillo, Carmelo Martín, Ariadna Prados Salazar, José Carlos Martín Ruiz, José Luis Genilloud, Olga Vicente, Francisca Pérez del Palacio, José Caba Pérez, Octavio Metabolomics Exocrine pancreatic insufficiency Chronic pancreatitis Biomarkers Diagnosis High-resolution mass spectrometry Background and Objectives: The clinical manifestations and course of chronic pancreatitis (CP) are often nonspecific and variable, hampering diagnosis of the risk of exocrine pancreatic insufficiency (EPI). Development of new, reproducible, and non-invasive methods to diagnose EPI is therefore a major priority. The objective of this metabolomic study was to identify novel biomarkers associated with EPI. Materials and Methods: We analyzed 53 samples from patients with CP, 32 with and 21 without EPI, using an untargeted metabolomics workflow based on hydrophilic interaction chromatography coupled to high-resolution mass spectrometry. Principal component and partial least squares-discriminant analyses showed significant between-group differentiation, and univariate and multivariate analyses identified potential candidate metabolites that significantly differed between samples from CP patients with EPI and those without EPI. Results: Excellent results were obtained using a six-metabolic panel to diagnose the presence of EPI in CP patients (area under the ROC curve = 0.785). Conclusions: This study confirms the usefulness of metabolomics in this disease setting, allowing the identification of novel biomarkers to differentiate between the presence and absence of EPI in CP patients. 2021-10-18T12:02:09Z 2021-10-18T12:02:09Z 2021 info:eu-repo/semantics/article Díaz, C.; Jiménez-Luna, C.; Diéguez-Castillo, C.; Martín, A.; Prados, J.; Martín-Ruíz, J.L.; Genilloud, O.; Vicente, F.; Palacio, J.P.d.; Caba, O. Untargeted Metabolomics for the Diagnosis of Exocrine Pancreatic Insufficiency in Chronic Pancreatitis. Medicina 2021, 57, 876. https://doi.org/10.3390/ medicina57090876 http://hdl.handle.net/10481/70940 10.3390/medicina57090876 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI