The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes Vallecillo Rivas, Marta Toledano Osorio, Manuel Vallecillo, Cristina Toledano Pérez, Manuel Osorio Ruiz, Raquel Bone regeneration Collagen membrane Hydrolytic degradation Collagen origin Bacterial collagenase Trypsin digestion Degradation testing Degradation kinetics This work was supported by: (1) the Ministry of Economy and Competitiveness and European Regional Development Fund [Project PID2020-114694RB-100 MINECO/AEI/FEDER/UE], (2) University of Granada/Regional Government of Andalusia Research Fund from Spain and European Regional Development Fund (A-BIO-157-UGR-18/FEDER). Collagen membranes are currently the most widely used membranes for guided bone regeneration; however, their rapid degradation kinetics means that the barrier function may not remain for enough time to permit tissue regeneration to happen. The origin of collagen may have an important effect on the resistance to degradation. The aim of this study was to investigate the biodegradation pattern of five collagen membranes from different origins: Biocollagen, Heart, Evolution X-fine, CopiOs and Parasorb Resodont. Membranes samples were submitted to different degradation tests: (1) hydrolytic degradation in phosphate buffer saline solution, (2) bacterial collagenase from Clostridium histolyticum solution, and (3) enzyme resistance using a 0.25% porcine trypsin solution. Immersion periods from 1 up to 50 days were performed. At each time point, thickness and weight measurements were performed with a digital caliper and an analytic microbalance, respectively. ANOVA and Student–Newman–Keuls tests were used for comparisons (p < 0.05). Differences between time-points within the same membranes and solutions were assessed by pair-wise comparisons (p < 0.001). The Evolution X-fine collagen membrane from porcine pericardium attained the highest resistance to all of the degradation tests. Biocollagen and Parasorb Resodont, both from equine origin, experienced the greatest degradation when immersed in PBS, trypsin and C. histolyticum during challenge tests. The bacterial collagenase solution was shown to be the most aggressive testing method. 2021-10-15T06:56:44Z 2021-10-15T06:56:44Z 2021-09-05 info:eu-repo/semantics/article Vallecillo-Rivas, M... [et al.]. The Collagen Origin Influences the Degradation Kinetics of Guided Bone Regeneration Membranes. Polymers 2021, 13, 3007. [https://doi.org/10.3390/polym13173007] http://hdl.handle.net/10481/70865 10.3390/polym13173007 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI