Identification of the thistle milk component Silibinin(A) and Glutathione-disulphide as potential inhibitors of the pancreatic lipase: Potential implications on weight loss Castillo Santaella, Teresa del Maldonado Valderrama, Julia Pancreatic lipase Silibinin(A) Obesity Lipolysis Emulsion Interfacial tension This work has been supported by Ministerio de Ciencia e Innovacion de Espana (under project RTI2018101309BC21) , by the Fundacion Seneca del Centro de Coordinacion de la Investigacion de la Region de Murcia (under Project 20988/PI/18) and by a grant from Ministerio de Economia y Competitividad de Espana (CTQ201787974R) . This research was partially supported by the supercomputing infrastructure of Poznan Supercomputing Centre, and by the einfrastructure program of the Research Council of Norway, and the supercomputer centre of UiTthe Arctic University of Norway. The authors also thankfully acknowledge the computer resources and the technical support provided by the Plataforma Andaluza de Bioinformatica of the University of Malaga. Powered@NLHPC: This research was partially supported by the supercomputing infrastructure of the NLHPC (ECM02) Peripheral targets like pancreatic-lipase appear to be the most suitable pharmacological alternative for obesity, as with orlistat, although its adverse effects limit its use. Therefore, the aim of this work was to identify new natural compounds able to inhibit pancreatic-lipase in an in vitro model. The DrugBank database was used to perform docking calculations. The best fitting-score compounds were further evaluated in vitro. Our data revealed that glutathione-disulphide (GSSG) and silibinin(A) inhibit pancreatic-lipase. This was confirmed by measuring hydrolysis in an emulsion model, obtaining that the suppression of lipid digestion by silibinin(A) was higher than that of GSSG and close to the effect of orlistat. Combined analysis established the existence of different inhibition mechanisms for each compound. In summary, silibinin(A) and GSSG inhibited pancreatic-lipase and, therefore, may be served as promise natural compounds to face with obesity. Further studies comprise the next step to fully validate the suitability of these compounds. 2021-10-08T08:50:29Z 2021-10-08T08:50:29Z 2021-08 journal article T. Del Castillo-Santaella et al. Identification of the thistle milk component Silibinin(A) and Glutathione-disulphide as potential inhibitors of the pancreatic lipase: Potential implications on weight loss. Journal of Functional Foods 83 (2021) 104479[https://doi.org/10.1016/j.jff.2021.104479] http://hdl.handle.net/10481/70744 10.1016/j.jff.2021.104479 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access Atribución-NoComercial-SinDerivadas 3.0 España Elsevier