Assessing the antioxidant and metabolic effect of an alpha-lipoic acid and acetyl-L-carnitine nutraceutical López Maldonado, Alicia Pastoriza de la Cueva, Silvia Rufián Henares, José Ángel Alpha lipoic acid Acetyl-L-carnitine Nutraceuticals Antioxidant Pilot study This work was supported by the research project Stance4Health funded by the European Union's Horizon 2020 research and innovation programme, grant agreement Nº. 816303 and by the Plan propio de Investigación y Transferencia of the University of Granada under the program “Intensificación de la Investigación, modalidad B”. This study was also supported by a Spanish predoctoral fellowship F.P.U. Nº 18/ 06440 from the Spanish Ministry of Science, Innovation and Universities. This paper will form part of the doctoral thesis by Alicia Lopez- Maldonado, conducted within the context of the “Clinical Medicine and Public Health Programme” at the University of Granada. Supplementary data to this article can be found online at https://doi.org/10.1016/j.crfs.2021.05.002. Personalized nutrition (PN) is seen as a potentially effective and affordable strategy for the prevention of non-communicable diseases (NCDs). In this study we aimed to evaluate the antioxidant and metabolic effect of a dietary supplement based on alpha-lipoic acid (ALA) and acetyl-L-carnitine (ALC) in order to include this product in a novel PN service. The antioxidant properties of the commercial nutraceutical were investigated at physiological conditions (through in vitro digestion) and at mitochondrial conditions. The metabolic activity was assessed in a human pilot study using a Gas Chromatography-Mass Spectrometry (GC-MS) methodology in dried urine samples. The nutraceutical exerted an elevated antiradical activity and reducing capacity, especially at mitochondrial conditions, after in vitro digestion. This increase in mitochondrial activity was also evidenced in vivo by a significant increase in the urinary phosphate concentration (p ​= ​0.004). As pro-oxidant effect was reached with the concentration of 4 capsules, 2 capsules at the same time could be a reasonable dose. No adverse effects were recorded in vivo with this dose. Thus, although its metabolic effect was not so conclusive, ALA ​+ ​ALC combination might be beneficial as a dietary supplement for the prevention of the oxidative stress and an interesting dietary supplement to consider in large scale studies. 2021-07-12T11:18:09Z 2021-07-12T11:18:09Z 2021 info:eu-repo/semantics/article A. Lopez-Maldonado et al. Assessing the antioxidant and metabolic effect of an alpha-lipoic acid and acetyl-L-carnitine nutraceutical. Current Research in Food Science 4 (2021) 336–344 [https://doi.org/10.1016/j.crfs.2021.05.002] http://hdl.handle.net/10481/69655 10.1016/j.crfs.2021.05.002 eng info:eu-repo/grantAgreement/EC/H2020/816303 http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España Elsevier