The Role of High-Resolution Analytical Techniques in the Development of Functional Foods Fernández Ochoa, Álvaro Leyva Jiménez, Francisco Javier Cádiz Gurrea, María de la Luz Pimentel Moral, Sandra Segura Carretero, Antonio Analytical techniques Analytical chemistry Chromatography Functional foods Mass spectrometry Nuclear magnetic resonance Metabolomics Phytochemicals This work was supported by the projects RTI2018-096724-B-C22 (Ministry of Science, Innovation and Universities) and P18-TP-3589 (Consejeria de Economia, Conocimiento, Empresas y Universidad from Junta de Andalucia). The author a Fernandez-Ochoa is grateful to the University of Granada for his post-doctoral contract ("Contrato Puente") during the years 2019-2020. The author FJ. Leyva-Jimenez received support from the Spanish Ministry of Economy and Competitiveness (MINECO) for the FPI grant BES-2016-076618 given to develop this work. The author M.d.l.L Cadiz-Gurrea is grateful to the Regional Ministry of Economy, Knowledge, Enterprise and Universities of Andalusia for the contract for Young Researchers (PAIDI) at the University of Granada. S. Pimentel-Moral is also grateful to the University of Granada for her postdoctoral contract. The approaches based on high-resolution analytical techniques, such as nuclear magnetic resonance or mass spectrometry coupled to chromatographic techniques, have a determining role in several of the stages necessary for the development of functional foods. The analyses of botanical extracts rich in bioactive compounds is one of the fundamental steps in order to identify and quantify their phytochemical composition. However, the compounds characterized in the extracts are not always responsible for the bioactive properties because they generally undergo metabolic reactions before reaching the therapeutic targets. For this reason, analytical techniques are also applied to analyze biological samples to know the bioavailability, pharmacokinetics and/or metabolism of the compounds ingested by animal or human models in nutritional intervention studies. In addition, these studies have also been applied to determine changes of endogenous metabolites caused by prolonged intake of compounds with bioactive potential. This review aims to describe the main types and modes of application of high-resolution analytical techniques in all these steps for functional food development. 2021-05-24T10:25:45Z 2021-05-24T10:25:45Z 2021-03-22 journal article Fernández-Ochoa, Á.; Leyva-Jiménez, F.J.; De la Luz Cádiz-Gurrea, M.; Pimentel-Moral, S.; Segura-Carretero, A. The Role of High-Resolution Analytical Techniques in the Development of Functional Foods. Int. J. Mol. Sci. 2021, 22, 3220. [https://doi.org/10.3390/ijms22063220] http://hdl.handle.net/10481/68676 10.3390/ijms22063220 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI