Reactive oxygen species (ROS) production in HepG2 cancer cell line through the application of localized alternating magnetic field Sola Leyva, Alberto Jabalera Ruz, Ylenia María Chico Lozano, María Ángeles Carrasco Jiménez, María Paz Iglesias Salto, Guillermo Ramón Jiménez López, Concepción This research work is supported by Ministerio de Economia y Competitividad (CGL2016-76723 project), Ramon y Cajal programme (RYC-2014-16901), Junta de Andalucia. Programa Operativo FEDER 2014-2020. (A1-FQM-341-UGR18, C-FQM-497-UGR18, A-BIO-376-UGR18). This research was also aided by the Andalusian regional government (CTS-236). Alberto Sola-Leyva holds a Formacion de Doctores 2018 grant (ref. PRE2018-085440) from the Ministerio de Ciencia, Innovacion y Universidades (Spain). Ylenia Jabalera wants to acknowledge a FPU2016 grant (ref. FPU16_04580) from the Ministerio de Educacion, Ciencia y Deporte y Competitividad (Spain) and Unidad Cientifica de Excelencia UCE-PP2016-05 of the University of Granada. This study is part of a PhD Thesis conducted at the University of Granada, Spain. Finally, thanks go to the CIC personnel of the University of Granada for technical assistance in the TEM. Recent studies have shown the potential of magnetic hyperthermia in cancer treatments. However, the underlying mechanisms involved have not been yet fully described. In particular, the cell death related to magnetic hyperthermia observed in cultures incubated with low concentration of magnetic nanoparticles and under a low intensity alternating magnetic field, in which a macroscopic temperature rise is not observed, is still not understood. In the present study, we investigate the production of intracellular Reactive Oxygen Species (ROS) as a mechanism to induce cell death under these conditions. In this study, the production and influence of ROS on the viability of HepG2 human hepatoma cells (used as a model cell line) are analyzed under the application of variable magnetic fields using hyperthermia agents, such as biomimetic magnetic nanoparticles (BMNPs) mediated by magnetosome MamC protein fromMagnetococcus marinusMC-1. The results show that intracellular ROS production increases up to similar to 90% following upon the exposure of AMF to HepG2 cells containing BMNPs, which could determine the loss of cell viability (up to similar to 40% reduction) without a significant rise in temperature. Such ROS production is linked to mitochondrial dysfunction caused by the application of AMF to cells containing BMNPs. 2021-05-20T10:36:37Z 2021-05-20T10:36:37Z 2020-07-24 info:eu-repo/semantics/article J. Mater. Chem. B, 2020, 8, 7667. [10.1039/d0tb01306d] http://hdl.handle.net/10481/68585 10.1039/d0tb01306d eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España Royal Society of Chemistry