Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors Hurtado Martínez, Miguel Muñoz Palazón, Barbara González Martínez, Alejandro Manzanera Ruiz, Maximino Enrique González López, Jesús Juan Pseudomonas Granular biomass Sequential batch reactor Nitrate-polluted groundwater Heterotrophic denitrification Four granular sequencing batch reactors (GSBRs) were inoculated with four denitrifying Pseudomonas strains carrying nosZ to study the process of granule formation, the operational conditions of the bioreactors, and the carbon concentration needed for nitrate removal. The selected Pseudomonas strains were P. stutzeri I1, P. fluorescens 376, P. denitrificans Z1, and P. fluorescens PSC26, previously reported as denitrifying microorganisms carrying the nosZ gene. Pseudomonas denitrificans Z1 produced fluffy, low-density granules, with a decantation speed below 10 m h−1 . However, P. fluorescens PSC26, P. stutzeri I1, and P. fluorescens 376 formed stable granules, with mean size from 7 to 15 mm, related to the strain and carbon concentration. P. stutzeri I1 and P. fluorescens 376 removed nitrate efficiently with a ratio in the range of 96%, depending on the source and concentration of organic matter. Therefore, the findings suggest that the inoculation of GSBR systems with denitrifying strains of Pseudomonas spp. containing the nosZ gene enables the formation of stable granules, the efficient removal of nitrate, and the transformation of nitrate into nitrogen gas, a result of considerable environmental interest to avoid the generation of nitrous oxide. 2021-05-14T11:58:29Z 2021-05-14T11:58:29Z 2021 info:eu-repo/semantics/article Hurtado-Martinez, M.; Muñoz-Palazon, B.; GonzalezMartinez, A.; Manzanera, M.; Gonzalez-Lopez, J. Groundwater Nitrate Removal Performance of Selected Pseudomonas Strains Carrying nosZ Gene in Aerobic Granular Sequential Batch Reactors. Water 2021, 13, 1119. https:// doi.org/10.3390/w13081119 http://hdl.handle.net/10481/68536 10.3390/w13081119 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI