Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study Llorente Cantarero, Francisco Jesús Anguita Ruiz, Augusto Mesa García, María Dolores Gil Hernández, Ángel Aguilera García, Concepción María Physical activity Accelerometry Oxidative stress Plasma total antioxidant capacity 8-hydroxy-20-deoxyguanosine isoprostane F2 The World Health Organization has recommended performing at least 60 min a day of moderate-to-vigorous physical activity (MVPA) and reducing sedentarism in children and adolescents to offer significant health benefits and mitigate health risks. Physical fitness and sports practice seem to improve oxidative stress (OS) status during childhood. However, to our knowledge, there are no data regarding the influence of objectively-measured physical activity (PA) and sedentarism on OS status in children and adolescents. The present study aimed to evaluate the influence of moderate and vigorous PA and sedentarism on OS and plasma total antioxidant capacity (TAC) in a selected Spanish population of 216 children and adolescents from the GENOBOX study. PA (light, moderate, and vigorous) and sedentarism (i.e., sedentary time (ST)) were measured by accelerometry. A Physical Activity-Sedentarism Score (PASS) was developed integrating moderate and vigorous PA and ST levels. Urinary 8-hydroxy-20-deoxyguanosine (8-OHdG) and isoprostane F2 (F2-IsoPs), as markers of OS, were determined by ELISA; and TAC was estimated by colorimetry using an antioxidant kit. A higher PASS was associated with lower plasma TAC and urinary 8-OHdG and F2-IsoPs, showing a better redox profile. Reduced OS markers (8-OHdG and F2-IsoPs) in children with higher PASS may diminish the need of maintaining high concentrations of antioxidants in plasma during rest to achieve redox homeostasis. 2021-03-23T10:24:19Z 2021-03-23T10:24:19Z 2021-02-20 info:eu-repo/semantics/article Llorente-Cantarero, F.J.; Aguilar-Gómez, F.J.; Leis, R.; Bueno, G.; Rupérez, A.I.; Anguita-Ruiz, A.; Vázquez-Cobela, R.; Mesa, M.D.; Moreno, L.A.; Gil, Á.; et al. Relationship between Physical Activity, Oxidative Stress, and Total Plasma Antioxidant Capacity in Spanish Children from the GENOBOX Study. Antioxidants 2021, 10, 320. [https://doi.org/10.3390/antiox10020320] http://hdl.handle.net/10481/67552 10.3390/antiox10020320 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Mdpi