Enzymatic Potential of Bacteria and Fungi Isolates from the Sewage Sludge Composting Process Robledo Mahón, Tatiana Calvo Sáinz, Concepción Aranda Ballesteros, Elisabet Bacteria Composting Enzymes Fungi Sewage sludge The aim of this study was the isolation and characterisation of the fungi and bacteria during the composting process of sewage sludge under a semipermeable membrane system at full scale, in order to find isolates with enzymatic activities of biotechnological interest. A total of 40 fungi were isolated and enzymatically analysed. Fungal culture showed a predominance of members of Ascomycota and Basidiomycota division and some representatives of Mucoromycotina subdivision. Some noticeable fungi isolated during the mesophilic and thermophilic phase were Aspergillus, Circinella, and Talaromyces. During the maturation phase, some lignin modifying enzyme producers, like Purpureocillium, Thielavia, Bjerkandera, or Dichotomyces, were found. Within this group, Thielavia and Bjerkandera showed high activity with production of laccases and peroxidases. In the bacterial culturome, a total of 128 strains were selected and enzymatically analysed. Bacillales, Actinomycetales, Pseudomonadales, and Lactobacillales were the orders most represented in culture-bacteria. Bacillus pumilus, B. stratosphericus, B. safensis, and Pseudomonas formosensis were the species most e cient in enzyme production, particularly peroxidases, polyphenol oxidases ammonifying activity, and amylases. These results showed that sewage sludge composting piles could represent a source of microorganisms which have adapted to adverse conditions. 2021-01-18T10:13:25Z 2021-01-18T10:13:25Z 2020-11-03 info:eu-repo/semantics/article Robledo-Mahón, T., Calvo, C., & Aranda, E. (2020). Enzymatic Potential of Bacteria and Fungi Isolates from the Sewage Sludge Composting Process. Applied Sciences, 10(21), 7763. [doi:10.3390/app10217763] http://hdl.handle.net/10481/65492 10.3390/app10217763 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Mdpi