Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels García Villén, Fátima Sánchez Espejo, Rita María Borrego Sánchez, Ana Viseras Iborra, César Antonio Sepiolite Palygorksite Spring water Hydrogel Wound healing Proliferation Franz cell Bioactive elements Inorganic hydrogels formulated with spring waters and clay minerals are used to treat musculoskeletal disorders and skin a ections. Their underlying mechanism of action for skin disorders is not clear, although it is usually ascribed to the chemical composition of the formulation. The aim of this study was to assess the composition and in vitro release of elements with potential wound healing e ects from hydrogels prepared with two nanoclays and natural spring water. In vitro Franz cell studies were used and the element concentration was measured by inductively coupled plasma techniques. Biocompatibility studies were used to evaluate the potential toxicity of the formulation against fibroblasts. The studied hydrogels released elements with known therapeutic interest in wound healing. The released ratios of some elements, such as Mg:Ca or Zn:Ca, played a significant role in the final therapeutic activity of the formulation. In particular, the proliferative activity of fibroblasts was ascribed to the release of Mn and the Zn:Ca ratio. Moreover, the importance of formulative studies is highlighted, since it is the optimal combination of the correct ingredients that makes a formulation e ective. 2020-12-14T12:17:24Z 2020-12-14T12:17:24Z 2020-09-18 info:eu-repo/semantics/article García-Villén, F., Sánchez-Espejo, R., Borrego-Sánchez, A., Cerezo, P., Cucca, L., Sandri, G., & Viseras, C. (2020). Correlation between Elemental Composition/Mobility and Skin Cell Proliferation of Fibrous Nanoclay/Spring Water Hydrogels. Pharmaceutics, 12(9), 891. [doi:10.3390/pharmaceutics12090891] http://hdl.handle.net/10481/64889 10.3390/pharmaceutics12090891 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Mdpi