LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma Arenas Molina, Alberto Manuel Cuadros Celorrio, Marta Eugenia Andrades Delgado, Álvaro García García, Daniel Jesús F. Coira, Isabel Rodríguez, María Isabel Baliñas Gavira, Carlos Peinado, Paola Álvarez Pérez, Juan Carlos P. Medina, Pedro Adenocarcinoma of lung Biomarkers Tumor RNA Long noncoding Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing roles in di erent pathologies including cancer. Over the last years, growing scientific evidence supports the key role of some lncRNAs in tumor development and proposes them as valuable biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA, DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines. We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity (AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential lung cancer biomarker. 2020-12-04T10:07:42Z 2020-12-04T10:07:42Z 2020-07-28 info:eu-repo/semantics/article Arenas, A. M., Cuadros, M., Andrades, A., García, D. J., Coira, I. F., Rodríguez, M. I., ... & Medina, P. P. (2020). LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma. Cancers, 12(8), 2080. [doi:10.3390/cancers12082080] http://hdl.handle.net/10481/64667 10.3390/cancers12082080 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Mdpi