Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming Lopez-Caraballo, Lidia Martorell Marugán, Jordi Carmona Sáez, Pedro González-Múñoz, Elena Molecular biology Stem Cells Research Transcriptomics We acknowledge the assistance and support of Laboratory for Cell Reprogramming and BIONAND students, colleagues, and collaborators. We thank members of the LARCEL laboratory and Prof. J.B. Cibelli (Michigan State University) for comments, discussion, and support; Dr. Ariane Wittgreen for intellectual input and discussion; and Biobanco del Sistema Sanitario Pu´blico de Andalucı´a for karyotyping and teratoma assay service. The authors thankfully acknowledge the computer resources (IPA software) provided by the PAB (Andalusian Bioinformatics Platform) center located at the University of Malaga (www.scbi.uma.es). Supplemental Information can be found online at https://doi.org/10.1016/j.isci.2020.101376. Cell reprogramming has revolutionized cell and regenerative biology field. However, human iPS derivation remains inefficient and variable. A better knowledge of molecular processes and the rationale underlying the importance of somatic cell origin is crucial to uncover reprogramming mechanisms. Here, we analyze the molecular profile of different human somatic cell types. We show menstrual blood-derived stromal cells (MnSCs) have a distinct, reprogramming prone, profile, and we identify SOX15 from their oocyte-related signature as a prominent responsible candidate. SOX15 orchestrates an efficient oocyte-based reprogramming combination when overexpressed with the also oocyte-enriched histone chaperone ASF1A and OCT4 and, through specific mechanism, generates iPSCs with distinguishable pluripotent state that further present higher differentiation capacity than canonical iPSCs. Our work supports the presence of different pluripotency states in reprogramming and the importance of using metaphase-II oocyte and MnSCs information to provide alternative reprogramming combinations and, importantly, to improve and understand pluripotency acquisition. 2020-11-17T09:43:29Z 2020-11-17T09:43:29Z 2020-08-21 journal article Lopez-Caraballo et al. Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming, iScience 23, 101376 [https://doi.org/10.1016/j.isci.2020.101376] http://hdl.handle.net/10481/64308 10.1016/j.isci.2020.101376 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access Atribución-NoComercial-SinDerivadas 3.0 España Cell Press; Elsevier