Nanoformulation Design Including MamC-Mediated Biomimetic Nanoparticles Allows the Simultaneous Application of Targeted Drug Delivery and Magnetic Hyperthermia Jabalera Ruz, Ylenia María Oltolina, Francesca Peigneux, Ana Sola Leyva, Alberto Carrasco Jiménez, María Paz Prat, María Jiménez López, Concepción Iglesias Salto, Guillermo Ramón Magnetic Hyperthermia Magnetic nanoparticles Biomimetic magnetic nanoparticles MamC Tumor combined dual targeting Doxorubicin G.R.I. wish to thank project RYC-2014-6901 (MINECO, Spain), Programa Operativo FEDER 2014-2020 (A1-FQM-341-UGR18, C-FQM-497-UGR18) Junta de Andalucia. C.J.-L. wants to thank projects CGL2016-76723 from the Ministerio de Economia y Competitividad from Spain and Fondo Europeo de Desarrollo Regional (FEDER) and Programa Operativo FEDER 2014-2020 (A-BIO-376-UGR18) Junta de Andalucia. M.P. was suppported by Progetto di Ricerca Fondi di Ateneo per la Ricerca-FAR 2017 "Development of innovative biological materials for the functional regeneration of cardiac tissue models". Y.J. wants to acknowledge a FPU2016 grant (ref. FPU16_04580) from the Ministerio de Educacion, Ciencia y Deporte y Competitividad (Spain). F.O. has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement Nffi 754446 and UGR Research and Knowledge Transfer Found-Athenea3i. A.S.-L. is funded by the Spanish Ministry of Science, Innovation and Universities (PRE2018-0854409) Thanks go to the Scientific Instrumentation Center (CIC) personnel of the University of Granada for technical assistance with the TEM. The following are available online at http://www.mdpi.com/2073-4360/12/8/1832/s1, Figure S1. Colloidal stability video of the samples measured using photograms of the sedimentation time evolution. The design of novel nanomaterials that can be used as multifunctional platforms allowing the combination of therapies is gaining increased interest. Moreover, if this nanomaterial is intended for a targeted drug delivery, the use of several guidance methods to increase guidance efficiency is also crucial. Magnetic nanoparticles (MNPs) allow this combination of therapies and guidance strategies. In fact, MNPs can be used simultaneously as drug nanocarriers and magnetic hyperthermia agents and, moreover, they can be guided toward the target by an external magnetic field and by their functionalization with a specific probe. However, it is difficult to find a system based on MNPs that exhibits optimal conditions as a drug nanocarrier and as a magnetic hyperthermia agent. In this work, a novel nanoformulation is proposed to be used as a multifunctional platform that also allows dual complementary guidance. This nanoformulation is based on mixtures of inorganic magnetic nanoparticles (M) that have been shown to be optimal hyperthermia agents, and biomimetic magnetic nanoparticles (BM), that have been shown to be highly efficient drug nanocarriers. The presence of the magnetosome protein MamC at the surface of BM confers novel surface properties that allow for the efficient and stable functionalization of these nanoparticles without the need of further coating, with the release of the relevant molecule being pH-dependent, improved by magnetic hyperthermia. The BM are functionalized with Doxorubicin (DOXO) as a model drug and with an antibody that allows for dual guidance based on a magnetic field and on an antibody. The present study represents a proof of concept to optimize the nanoformulation composition in order to provide the best performance in terms of the magnetic hyperthermia agent and drug nanocarrier. 2020-11-17T08:44:17Z 2020-11-17T08:44:17Z 2020-08-15 info:eu-repo/semantics/article Jabalera, Y.; Oltolina, F.; Peigneux, A.; Sola-Leyva, A.; Carrasco-Jiménez, M.P.; Prat, M.; Jimenez-Lopez, C.; Iglesias, G.R. Nanoformulation Design Including MamC-Mediated Biomimetic Nanoparticles Allows the Simultaneous Application of Targeted Drug Delivery and Magnetic Hyperthermia. Polymers 2020, 12, 1832. [doi:10.3390/polym12081832] http://hdl.handle.net/10481/64304 10.3390/polym12081832 eng info:eu-repo/grantAgreement/EU/H2020/Nffi754446 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI