Computational Methods and Software Tools for Functional Analysis of miRNA Data García Moreno, Adrian Carmona Sáez, Pedro Functional analysis miRNAs ncRNA Databases Enrichment Tools miRNAs are important regulators of gene expression that play a key role in many biological processes. High-throughput techniques allow researchers to discover and characterize large sets of miRNAs, and enrichment analysis tools are becoming increasingly important in decoding which miRNAs are implicated in biological processes. Enrichment analysis of miRNA targets is the standard technique for functional analysis, but this approach carries limitations and bias; alternatives are currently being proposed, based on direct and curated annotations. In this review, we describe the two workflows of miRNAs enrichment analysis, based on target gene or miRNA annotations, highlighting statistical tests, software tools, up-to-date databases, and functional annotations resources in the study of metazoan miRNAs. 2020-11-12T09:59:51Z 2020-11-12T09:59:51Z 2020 info:eu-repo/semantics/article Garcia-Moreno, Adrian; Carmona-Saez, Pedro. 2020. "Computational Methods and Software Tools for Functional Analysis of miRNA Data." Biomolecules 10, no. 9: 1252. [doi: 10.3390/biom10091252] http://hdl.handle.net/10481/64217 10.3390/biom10091252 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI