Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery García Pinel, Beatriz Cabeza, Laura Ortiz, Raúl Prados Salazar, José Carlos Melguizo Alonso, Consolación PNIPAm Nanosystems Magnetic nanoparticle Fluorouracil; oxaliplatin Colon cancer External magnetic field Here, temperature-sensitive hybrid poly(N-isopropylacrylamide) (pNIPAM) nanosystems with magnetic response are synthesised and investigated for controlled release of 5-fluorouracil (5FU) and oxaliplatin (OXA). Initially, magnetic nanoparticles (@Fe3O4) are synthesised by co-precipitation approach and functionalised with acrylic acid (AA), 3-butenoic acid (3BA) or allylamine (AL) as comonomers. The thermoresponsive polymer is grown by free radical polymerisation using N-isopropylacrylamide (NIPAM) as monomer, N,N’-methylenbisacrylamide (BIS) as cross-linker, and 2,2’-azobis(2-methylpropionamidene) (V50) as initiator. We evaluate particle morphology by transmission electron microscopy (TEM) and particle size and surface charge by dynamic light scattering (DLS) and Z-potential (ZP) measurements. These magnetically active pNIPAM@ nanoformulations are loaded with 5-fluorouracil (5FU) and oxaliplatin (OXA) to determine loading efficiency, drug content and release as well as the cytotoxicity against T-84 colon cancer cells. Our results show high biocompatibility of pNIPAM nanoformulations using human blood cells and cultured cells. Interestingly, the pNIPAM@Fe3O4-3BA þ 5FU nanoformulation significantly reduces the growth of T-84 cells (57% relative inhibition of proliferation). Indeed, pNIPAM-co-AL@Fe3O4-AA nanosystems produce a slight migration of HCT15 cells in suspension in the presence of an external magnetic field. Therefore, the obtained hybrid nanoparticles can be applied as a promising biocompatible nanoplatform for the delivery of 5FU and OXA in the improvement of colon cancer treatments. 2020-11-10T11:05:47Z 2020-11-10T11:05:47Z 2020-01-01 info:eu-repo/semantics/article Garcia-Pinel, B., Ortega-Rodríguez, A., Porras-Alcalá, C., Cabeza, L., Contreras-Cáceres, R., Ortiz, R., ... & López-Romero, J. M. (2020). Magnetically active pNIPAM nanosystems as temperature-sensitive biocompatible structures for controlled drug delivery. Artificial Cells, Nanomedicine, and Biotechnology, 48(1), 1022-1035. [https://doi.org/10.1080/21691401.2020.1773488] http://hdl.handle.net/10481/64165 10.1080/21691401.2020.1773488 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Taylor y Francis LTD