Comparative Study of the Antioxidant and Anti-Inflammatory E ects of Leaf Extracts from Four Di erent Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice Leyva Jiménez, Francisco Javier Ruiz Malagón, Antonio Jesús Molina Tijeras, José Alberto Díez Echave, Patricia Vezza, Teresa Hidalgo García, Laura Lozano Sánchez, Jesús Arráez Román, David Rodríguez Nogales, Alba Segura Carretero, Antonio Gálvez Peralta, Julio Juan Morus alba leaves High fat diet Obesity Chemical characterization Anti-inflammatory The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Increased levels of reactive oxygen species (ROS) and a low-grade chronic inflammation in multiple organs have been demonstrated in obesity. Morus alba leaves extracts (MAEs) have been used in traditional medicine as anti-inflammatory agents. In this work, the bioactive compounds of di erent genotypes of M. alba L. (Filipina, Valenciana Temprana, Kokuso, and Italia) were analyzed not only by reverse phase high performance liquid chromatography–electrospray ionization-time of flight-mass spectrometry (RP-HPLC-ESI-TOF-MS) and hydrophilic interaction chromatography–electrospray ionization-time of flight-mass spectrometry (HILIC-ESI-TOF-MS), but also screened for in vitro and in vivo antioxidant activity by means of DPPH radical scavenging assay and Caenorhabditis elegans model. These MAEs were administered daily in a model of diet-induced obesity in mice. Filipina and Italia genotypes significantly reduced weight gain, the glycemic levels in high fat diet, as well as, levels of LDL-cholesterol and triglycerides. Filipina and Italia MAEs also reduced the expression of proinflammatory mediators such as Tnf- , Il-1 , Il-6 and increased the levels of adiponectin and AMPK, which exert anti-inflammatory e ects. Moreover, Italia genotype ameliorated the intestinal barrier function. In conclusion, Filipina and Italia methanolic extracts show the highest antioxidant and anti-inflammatory e ect, due to the presence of compounds such as protocatechuic acid or quercetin-3-glucoside, and they could be developed as a complementary treatment for obesity and metabolic disorders. 2020-11-04T12:37:39Z 2020-11-04T12:37:39Z 2020-08-11 info:eu-repo/semantics/article Leyva-Jiménez, F. J., Ruiz-Malagón, A. J., Molina-Tijeras, J. A., Diez-Echave, P., Vezza, T., Hidalgo-García, L., ... & Rodríguez-Nogales, A. (2020). Comparative Study of the Antioxidant and Anti-Inflammatory Effects of Leaf Extracts from Four Different Morus alba Genotypes in High Fat Diet-Induced Obesity in Mice. Antioxidants, 9(8), 733. [doi:10.3390/antiox9080733] http://hdl.handle.net/10481/64056 10.3390/antiox9080733 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Mdpi