Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides Verni, Michela Verardo, Vito Díaz-de-Cerio, Elixabet Brewers’ spent grains Bioprocessing Phenolic compounds Bioactive peptides Antioxidant activity This research was developed under the project "FUNBREW Biotransformation of brewers' spent grain: increased functionality for novel food applications" funded by the European Union's Horizon 2020 Research and Innovation Program under grant agreement No. 727473 (ERANet SUSFOOD2). Brewers’ spent grain (BSG) is the major by-product of the brewing industry which remain largely unutilized despite its nutritional quality. In this study, the effects of fermentation on BSG antioxidant potential were analyzed. A biotechnological protocol including the use of xylanase followed by fermentation with Lactiplantibacillus plantarum (Lactobacillus plantarum) PU1, PRO17, and H46 was used. Bioprocessed BSG exhibited enhanced antioxidant potential, characterized by high radical scavenging activity, long-term inhibition of linoleic acid oxidation and protective effect toward oxidative stress on human keratinocytes NCTC 2544. Immunolabelling and confocal laser microscopy showed that xylanase caused an extensive cell wall arabinoxylan disruption, contributing to the release of bound phenols molecules, thus available to further conversion through lactic acid bacteria metabolism. To clarify the role of fermentation on the antioxidant BSG potential, phenols were selectively extracted and characterized through HPLC-MS techniques. Novel antioxidant peptides were purified and identified in the most active bioprocessed BSG. 2020-09-25T12:39:00Z 2020-09-25T12:39:00Z 2020-07-31 info:eu-repo/semantics/article Verni M, Pontonio E, Krona A, Jacob S, Pinto D, Rinaldi F, Verardo V, Díaz-de-Cerio E, Coda R and Rizzello CG (2020) Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides. Front. Microbiol. 11:1831. [doi: 10.3389/fmicb.2020.01831] http://hdl.handle.net/10481/63555 10.3389/fmicb.2020.01831 eng EU/H2020/727473 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Frontiers Media