Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration Toledano Pérez, Manuel Asady, Samara Toledano Osorio, Manuel Benítez García, José A. Osorio Ruiz, Raquel Collagen Membranes Biodegradation Bone regeneration Native collagen-based membranes are used to guide bone regeneration; but due to their rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm2 pieces of membranes were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial (Clostridium histolyticum) collagenase resistance test. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes’ images. ANOVA and Student Newman Keuls were used for mean comparisons (p < 0.05), except when analyzing differences between time-points within the same membrane and solution where pair-wise comparisons were applied (p < 0.001). Derma Fina attained the highest resistance to all degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most aggressive test as all membranes presented 100% degradation before 21 d. 2020-09-14T08:44:54Z 2020-09-14T08:44:54Z 2020-06-04 journal article Toledano, M., Asady, S., Toledano-Osorio, M., García-Godoy, F., Serrera-Figallo, M. A., Benítez-García, J. A., & Osorio, R. (2020). Differential Biodegradation Kinetics of Collagen Membranes for Bone Regeneration. Polymers, 12(6), 1290. [doi:10.3390/polym12061290] http://hdl.handle.net/10481/63407 doi:10.3390/polym12061290 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI