Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage Taamalli, Amani Lozano Sánchez, Jesús Segura Carretero, Antonio Arráez Román, David Olive leaves Supercritical fluid extraction Antioxidants Virgin olive oil has demonstrated its effective activity against oxidative stress. However, data on the bioactive effect of olive leaves or their major constituents on the liver are scarce. The present research work was conducted to evaluate the hepatoprotective effects of supercritical carbon dioxide (SC-CO2) extracts from fresh and dried olive leaves on hepatotoxicity caused by carbon tetrachloride (CCl4) in rat models. For this purpose, healthy albino rats of 180–250 g weight were used. The assessment of biochemical markers was carried out on blood and liver tissue. Then, a histopathological study was carried out on liver tissue. The obtained results showed that fresh and dried olive leaf extracts ameliorate the perturbed biochemical parameters caused by CCl4 treatment. Furthermore, the results registered for the histopathological study are in accordance with the biochemical parameters and the protective capacity of SC-CO2 extracts against DNA damage, indicating that olive leaf extracts helped to improve liver fibrosis caused by CCl4 treatment. 2020-09-11T07:37:18Z 2020-09-11T07:37:18Z 2020-06-18 info:eu-repo/semantics/article Taamalli, A., Feriani, A., Lozano-Sanchez, J., Ghazouani, L., El Mufti, A., Allagui, M. S., ... & Arráez-Roman, D. (2020). Potential Hepatoprotective Activity of Super Critical Carbon Dioxide Olive Leaf Extracts against CCl4-Induced Liver Damage. Foods, 9(6), 804. [doi:10.3390/foods9060804] http://hdl.handle.net/10481/63379 10.3390/foods9060804 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI