Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin Toledano Pérez, Manuel Sánchez Aguilera, Fátima Cabello, Inmaculada Toledano Osorio, Manuel Osorio Ruiz, María Estrella López López, Modesto Torcuato García-Godoy, Franklin Lynch, Christopher D. Osorio Ruiz, Raquel Dentin Mechanical Mineralization Roughness Viscoelastic Background: The aim was to evaluate the effect of silver loaded nanoparticles (NPs) application on the triboscopic, crystallographic and viscoelastic properties of demineralized dentin. Polymethylmetacrylate-based NPs and Ag loaded NPs were applied on demineralized dentin. Material and Methods: Treated and untreated surfaces were probed by a nanoindenter to test viscoelasticity, and by atomic force microscopy to test nanoroughness and collagen fibril diameter. X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-field imaging were also used. Results: Dentin treated with Ag-NPs attained the lowest complex modulus, and the highest tan delta values after 7 days of storage. Dentin treated with undoped-NPs achieved the lowest nanoroughness and the greatest collagen bandwidths among groups. Crystals were identified as hydroxyapatite with the highest crystallographic maturity and crystallite size in dentin treated with undoped-NPs. Texture increased in all samples from 24 h to 7 d, except in dentin surfaces treated with Ag-NPs at 310 plane. Polyhedral, block-like, hexagonal or plate-like shaped apatite crystals constituted the bulk of minerals in dentin treated with Ag-NPs, after 7 d. Polyhedral or rounded/drop-like, and polymorphic in strata crystal apatite characterized the minerals when undoped-NPs were used, with more crystalline characteristics after 7 d than that found when Ag-NPs were applied. Ag-NPs application did not improve the mechanical performance of dentin and did not produce dentin remineralization. However, energy was dissipated through the dentin without showing stress concentration; contrary was occurring at dentin treated with undoped- NPs, that provoked bridge-like mineral deposits at the dentin surface. Conclusions: Ag-NPs application did not enhance the mechanical properties of cervical dentin, though the energy dissipation did not damage the dentin structure. Remineralization at dentin was not produced after Ag-NPs application, though improved crystallinity may lead to increase stability of the apatite that was generated at the dentin surface. 2020-04-27T11:42:08Z 2020-04-27T11:42:08Z 2019 journal article Toledano M, Aguilera FS, Cabello I, Toledano-Osorio M, Osorio E, López-López MT, García-Godoy F, Lynch CD, Osorio R. Silver-loaded nanoparticles affect ex-vivo mechanical behavior and mineralization of dentin. Med Oral Patol Oral Cir Bucal. 2019 Mar 1;24 (2):e156-64. http://hdl.handle.net/10481/61611 10.4317/medoral.22885 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access Atribución-NoComercial-SinDerivadas 3.0 España Medicina Oral Sl