Formulation, Colloidal Characterization, and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration Castillo Santaella, Teresa del Ortega-Oller, Inmaculada Padial Molina, Miguel O'Valle Ravassa, Francisco Javier Galindo Moreno, Pablo Antonio Jódar Reyes, Ana Belén Peula-García, José Manuel BMP-2 PLGA nanoparticles Pluronic F68 The following are available online at https://www.mdpi.com/1999-4923/11/8/388/s1, Figure S1. Scheme of the formulation of NP-BMP2; Figure S2: Scheme of the protein adsorption process for NP-BSA-BMP2; Video S1. NTA experiments for NP-BMP2; Video S2. NTA experiments for empty NPs. Nanoparticles (NPs) based on the polymer poly (lactide-co-glycolide) acid (PLGA) have been widely studied in developing delivery systems for drugs and therapeutic biomolecules, due to the biocompatible and biodegradable properties of the PLGA. In this work, a synthesis method for bone morphogenetic protein (BMP-2)-loaded PLGA NPs was developed and optimized, in order to carry out and control the release of BMP-2, based on the double-emulsion (water/oil/water, W/O/W) solvent evaporation technique. The polymeric surfactant Pluronic F68 was used in the synthesis procedure, as it is known to have an effect on the reduction of the size of the NPs, the enhancement of their stability, and the protection of the encapsulated biomolecule. Spherical solid polymeric NPs were synthesized, showing a reproducible multimodal size distribution, with diameters between 100 and 500 nm. This size range appears to allow the protein to act on the cell surface and at the cytoplasm level. The effect of carrying BMP-2 co-adsorbed with bovine serum albumin on the NP surface was analyzed. The colloidal properties of these systems (morphology by SEM, hydrodynamic size, electrophoretic mobility, temporal stability, protein encapsulation, and short-term release profile) were studied. The effect of both BMP2-loaded NPs on the proliferation, migration, and osteogenic differentiation of mesenchymal stromal cells from human alveolar bone (ABSC) was also analyzed in vitro. 2020-04-22T13:02:18Z 2020-04-22T13:02:18Z 2019-08-03 info:eu-repo/semantics/article del Castillo-Santaella, T.; Ortega-Oller, I.; Padial-Molina, M.; O’Valle, F.; Galindo-Moreno, P.; Jódar-Reyes, A.B.; Peula-García, J.M. Formulation, Colloidal Characterization, and In Vitro Biological Effect of BMP-2 Loaded PLGA Nanoparticles for Bone Regeneration. Pharmaceutics 2019, 11, 388. [doi:10.3390/pharmaceutics11080388] http://hdl.handle.net/10481/61504 10.3390/pharmaceutics11080388 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI