<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/61492">
      <dc:title>Pancreatic (pro)enzymes treatment suppresses BXPC-3 pancreatic Cancer Stem Cell subpopulation and impairs tumour engrafting</dc:title>
      <dc:creator>Hernández Camarero, Pablo</dc:creator>
      <dc:creator>López Ruiz, Elena</dc:creator>
      <dc:creator>Griñán Lisón, Carmen</dc:creator>
      <dc:creator>García Chaves, María Ángel</dc:creator>
      <dc:creator>Chocarro Wrona, Carlos</dc:creator>
      <dc:creator>Marchal Corrales, Juan Antonio</dc:creator>
      <dc:creator>Kenyon, Julian</dc:creator>
      <dc:creator>Perán, Macarena</dc:creator>
      <dc:description>Cancer stem cells (CSCs) subpopulation within the tumour is responsible for metastasis and cancer&#xd;
relapse. Here we investigate in vitro and in vivo the effects of a pancreatic (pro)enzyme mixture&#xd;
composed of Chymotrypsinogen and Trypsinogen (PRP) on CSCs derived from a human pancreatic&#xd;
cell line, BxPC3. Exposure of pancreatic CSCs spheres to PRP resulted in a significant decrease of&#xd;
ALDEFLUOR and specific pancreatic CSC markers (CD 326, CD 44 and CxCR4) signal tested by flow&#xd;
cytometry, further CSCs markers expression was also analyzed by western and immunofluorescence&#xd;
assays. PRP also inhibits primary and secondary sphere formation. Three RT2 Profiler PCR Arrays&#xd;
were used to study gene expression regulation after PRP treatment and resulted in, (i) epithelialmesenchymal&#xd;
transition (EMT) inhibition; (ii) CSCs related genes suppression; (iii) enhanced expression&#xd;
of tumour suppressor genes; (iv) downregulation of migration and metastasis genes and (v) regulation&#xd;
of MAP Kinase Signalling Pathway. Finally, in vivo anti-tumor xenograft studies demonstrated high&#xd;
anti-tumour efficacy of PRP against tumours induced by BxPC3 human pancreatic CSCs. PRP impaired&#xd;
engrafting of pancreatic CSC’s tumours in nude mice and displayed an antigrowth effect toward&#xd;
initiated xenografts. We concluded that (pro)enzymes treatment is a valuable strategy to suppress the&#xd;
CSC population in solid pancreatic tumours.</dc:description>
      <dc:date>2020-04-22T11:44:31Z</dc:date>
      <dc:date>2020-04-22T11:44:31Z</dc:date>
      <dc:date>2019-08-06</dc:date>
      <dc:type>info:eu-repo/semantics/article</dc:type>
      <dc:identifier>Hernández-Camarero, P., López-Ruiz, E., Griñán-Lisón, C., García, M. Á., Chocarro-Wrona, C., Marchal, J. A., ... &amp; Perán, M. (2019). Pancreatic (pro) enzymes treatment suppresses BXPC-3 pancreatic Cancer Stem Cell subpopulation and impairs tumour engrafting. Scientific reports, 9(1), 1-17.</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/61492</dc:identifier>
      <dc:identifier>10.1038/s41598-019-47837-7</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>Springer Nature</dc:publisher>
   </ow:Publication>
</rdf:RDF>