Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression Melguizo Rodríguez, Lucía Raquel Manzano Moreno, Francisco Javier Illescas Montes, Rebeca Ramos Torrecillas, Javier Luna Bertos, María Elvira De Ruiz Rodríguez, Concepción García Martínez, Olga Extra-virgin olive oil Phenolic compounds Gene expression Genetic profile The phenolic compounds of extra-virgin olive oil can act at various levels to protect individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with 10-6 M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin. The expression of studied markers was quantified using quantitative real-time polymerase chain reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation markers was modified after 24 h of treatment with 10-6 M of the phenolic compounds under study, most of which increased the gene expression of the transforming growth factor 1 (TGF- 1), TGF- receptor 1,2 and 3 (TGF- R1, TGF- R2, TGF- R3), bone morphogenetic protein 2 and 7 (BMP2, BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC), Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would support their protective effect against bone pathologies. 2020-04-16T11:57:49Z 2020-04-16T11:57:49Z 2019-07-25 info:eu-repo/semantics/article Melguizo-Rodríguez, L., Manzano-Moreno, F. J., Illescas-Montes, R., Ramos-Torrecillas, J., de Luna-Bertos, E., Ruiz, C., & García-Martínez, O. (2019). Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression. Nutrients, 11(8), 1722. http://hdl.handle.net/10481/61301 10.3390/nu11081722 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI