Occurrence of Mycotoxins in Swine Feeding from Spain Arroyo-Manzanares, Natalia Rodríguez Estévez, Vicente Arenas Fernández, Plácido García Campaña, Ana María Gámiz Gracia, Laura Liquid chromatography Fluorescence detection Mass spectrometry Solid-liquid extraction Feed A survey including 228 pig feed samples from Spain has been developed, exploring the occurrence of 19 mycotoxins (aflatoxins B1, B2, G1 and G2, ochratoxin A, fumonisins B1 and B2, citrinin, zearalenone, deoxynivalenol, fusarenon X, sterigmatocystin, T-2 toxin, HT-2 toxin, enniatins A, A1, B and B2, and beauvericin). The samples were analysed by solid-liquid extraction followed by liquid chromatography coupled with fluorescence or mass spectrometry detection. Enniatin B was found in 100% of the samples (up to 1200 ug/kg) and beauvericin in more than 90%. Moreover, 40% of samples were contaminated with more than five mycotoxins. This high occurrence is insurmountable and surpasses all previous studies, probably due to the inclusion of emerging mycotoxins, scarcely explored. The majority of the samples (96.9%) were in accordance with EU regulations, which do not address emerging mycotoxins or co-occurrence. These results show that in order to ensure mycotoxin absence, emerging mycotoxins should always be considered. 2020-03-10T11:55:56Z 2020-03-10T11:55:56Z 2019-06-15 info:eu-repo/semantics/article Arroyo-Manzanares, N., Rodríguez-Estévez, V., Arenas-Fernández, P., García-Campaña, A. M., & Gámiz-Gracia, L. (2019). Occurrence of Mycotoxins in Swine Feeding from Spain. Toxins, 11(6), 342. http://hdl.handle.net/10481/60187 10.3390/toxins11060342 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI