<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/58609">
      <dc:title>Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans</dc:title>
      <dc:creator>Toledano Pérez, Manuel</dc:creator>
      <dc:creator>Toledano Osorio, Manuel</dc:creator>
      <dc:creator>Navarro Hortal, María Dolores</dc:creator>
      <dc:creator>Varela López, Alfonso</dc:creator>
      <dc:creator>Osorio Ruiz, Raquel</dc:creator>
      <dc:creator>Quiles Morales, José Luis</dc:creator>
      <dc:subject>Dentistry</dc:subject>
      <dc:subject>Doxycycline</dc:subject>
      <dc:subject>Fertility</dc:subject>
      <dc:subject>Growth</dc:subject>
      <dc:subject>Lethality</dc:subject>
      <dc:subject>Metabolism</dc:subject>
      <dc:subject>Nanoparticles</dc:subject>
      <dc:description>The objective was to evaluate the toxicity of zinc- and doxycycline-loaded polymeric&#xd;
nanoparticles (NPs) using Caenorhabditis elegans as a model organism. These NPs are composed&#xd;
of ethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacrylic acid. NPs were&#xd;
loaded with doxycycline (D-NPs) and zinc (Zn-NPs) by chemical adsorption, and loading efficacy was&#xd;
demonstrated. Worm death rate in a concentration-response curve basis was calculated for lethality.&#xd;
Metabolism was evaluated through pharyngeal pumping assay. Body length measurements, brood&#xd;
size and egg lays were used to gauge growth, reproduction and fertility respectively. Intracellular&#xd;
hydrogen peroxide levels were determined to assess the reactive oxygen species production. One-way&#xd;
ANOVA and Bonferroni were used for comparisons (p &lt; 0.05). Tested NPs at the highest dosage did&#xd;
not affect lethality or worm metabolism, expressed in terms of death rate and pharyngeal pumping per&#xd;
minute, respectively. Zn-NPs slightly increased worm growth. The concentration of the intracellular&#xd;
hydrogen peroxide levels was the lowest in the D-NPs group. The distinct NPs and concentrations&#xd;
employed were shown to be non-toxic for in situ administration of zinc and doxycycline, reducing&#xd;
the harmful effects of these compounds.</dc:description>
      <dc:date>2020-01-09T13:47:49Z</dc:date>
      <dc:date>2020-01-09T13:47:49Z</dc:date>
      <dc:date>2019-11-14</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Toledano, M., Toledano-Osorio, M., Navarro-Hortal, M. D., Varela-López, A., Osorio, R., &amp; Quiles, J. L. (2019). Novel Polymeric Nanocarriers Reduced Zinc and Doxycycline Toxicity in the Nematode Caenorhabditis elegans. Antioxidants, 8(11), 550.</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/58609</dc:identifier>
      <dc:identifier>10.3390/antiox8110550</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>MDPI</dc:publisher>
   </ow:Publication>
</rdf:RDF>