Tumor Targeting by Monoclonal Antibody Functionalized Magnetic Nanoparticles Oltolina, Francesca Magnetic nanoparticles Tumor targeting Monoclonal antibodies Cytotoxicity Doxorubicin Tumor-targeted drug-loaded nanocarriers represent innovative and attractive tools for cancer therapy. Several magnetic nanoparticles (MNPs) were analyzed as potential tumor-targeted drug-loaded nanocarriers after functionalization with anti-Met oncogene (anti-Met/HGFR) monoclonal antibody (mAb) and doxorubicin (DOXO). Their cytocompatibility, stability, immunocompetence (immunoprecipitation), and their interactions with cancer cells in vitro (Perl’s staining, confocal microscopy, cytotoxic assays: MTT, real time toxicity) and with tumors in vivo (Perl’s staining) were evaluated. The simplest silica- and calcium-free mAb-loaded MNPs were the most cytocompatible, the most stable, and showed the best immunocompetence and specificity. These mAb-functionalized MNPs specifically interacted with the surface of Met/HGFR-positive cells, and not with Met/HGFR-negative cells; they were not internalized, but they discharged in the targeted cells DOXO, which reached the nucleus, exerting cytotoxicity. The presence of mAbs on DOXO-MNPs significantly increased their cytotoxicity on Met/HGFR-positive cells, while no such effect was detectable on Met/HGFR-negative cells. Bare MNPs were biocompatible in vivo; mAb presence on MNPs induced a better dispersion within the tumor mass when injected in situ in Met/HGFR-positive xenotumors in NOD/SCID-y null mice. These MNPs may represent a new and promising carrier for in vivo targeted drug delivery, in which applied gradient and alternating magnetic fields can enhance targeting and induce hyperthermia respectively. 2020-01-09T13:45:59Z 2020-01-09T13:45:59Z 2019-11-06 journal article Oltolina, F., Colangelo, D., Miletto, I., Clemente, N., Miola, M., Verné, E., ... & Follenzi, A. (2019). Tumor Targeting by Monoclonal Antibody Functionalized Magnetic Nanoparticles. Nanomaterials, 9(11), 1575. http://hdl.handle.net/10481/58585 10.3390/nano9111575 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI