Increased Expression of Musashi-1 Evidences Mesenchymal Repair in Maxillary Sinus Floor Elevation O'Valle Ravassa, Francisco Javier Buitrago, Juan G. de Hernández Cortés, Pedro Manuel Padial Molina, Miguel Crespo-Lora, Vicente Cobo, Marien Aguilar Peña, David Galindo Moreno, Pablo Antonio This study aimed to analyze the expression of Musashi-1 (MSI1) in maxillary native bone and grafted bone after maxillary sinus floor elevation. To do so, fifty-seven bone biopsies from 45 participants were studied. Eighteen samples were collected from native bone while 39 were obtained 6 months after maxillary sinus grafting procedures. Musashi-1 was analyzed by immunohistochemistry and RT-PCR. MSI1 was detected in osteoblasts and osteocytes in 97.4% (38/39) of grafted areas. In native bone, MSI1 was detected in only 66.6% (12/18) of the biopsies, mainly in osteocytes. Detection of MSI1 was significantly higher in osteoprogenitor mesenchymal cells of grafted biopsies (p < 0.001) but minor in smooth muscle and endothelial cells; no expression was detected in adipocytes. The mesenchymal cells of the non-mineralized tissue of native bone showed very low nuclear expression of MSI1, in comparison to fusiform cells in grafted areas (0.28(0.13) vs. 2.10(0.14), respectively; p < 0.001). Additionally, the detection of MSI1 mRNA was significantly higher in biopsies from grafted areas than those from native bone (1.00(0.51) vs. 60.34(35.2), respectively; p = 0.029). Thus, our results regardig the significantly higher detection of Musashi-1 in grafted sites than in native bone reflects its importance in the remodeling/repair events that occur after maxillary sinus floor elevation in humans. 2019-11-27T11:52:52Z 2019-11-27T11:52:52Z 2018-08-16 info:eu-repo/semantics/article O’Valle, F., de Buitrago, J. G., Hernández-Cortés, P., Padial-Molina, M., Crespo-Lora, V., Cobo, M., ... & Galindo-Moreno, P. (2018). Increased expression of Musashi-1 evidences mesenchymal repair in maxillary sinus floor elevation. Scientific reports, 8(1), 12243. http://hdl.handle.net/10481/58093 10.1038/s41598-018-29908-3 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Springer Nature