Microbial Production of Ethanol From Sludge Derived From an Urban Wastewater Treatment Plant Godoy, Patricia Mourenza, Álvaro Hernández Romero, Sergio Gonzalez-Lopez, Jesus Manzanera Ruiz, Maximino Enrique Microethanol Wastewater sludge Proteus Biofuel Wastewater treatment Residue valorization A collection of lipase-producing microorganisms was isolated from sludge derived from an urban wastewater treatment plant. The microorganisms with the highest levels of lipase activity were selected in order to use triglycerides present in the sludge effectively and were then transformed with pdc:adhB genes for the production of ethanol. The transgenic strains showed high growth rates in diluted sludge and produced lipase protein in order to utilize fat present in the sludge, which provides an abundant source of carbon. Using sludge derived from treated wastewater as nutrient source, ethanol was produced by certain transgenic species belonging to the genera Proteus. Different forms of sludge were tested for maximal ethanol production, with dehydrated sludge being found to produce the best performance. 2019-11-05T12:32:16Z 2019-11-05T12:32:16Z 2018-11-01 info:eu-repo/semantics/article Godoy P, Mourenza Á, Hernández-Romero S, González-López J and Manzanera M (2018) Microbial Production of Ethanol From Sludge Derived From an Urban Wastewater Treatment Plant. Front. Microbiol. 9:2634. http://hdl.handle.net/10481/57696 10.3389/fmicb.2018.02634 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España Frontiers Media