Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases Fernández Ochoa, Álvaro Quirantes-Piné, Rosa Borras Linares, María Isabel Segura-Carrettero, Antonio Alarcón Riquelme, Marta Eugenia Microbiomic Metabolomics Systemic autoimmune diseases Systemic lupus erythematosus Sjögren’s syndrome Primary anti-phosholipid syndrome Undifferentiated connective tissue diseases Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs. 2019-10-31T12:11:35Z 2019-10-31T12:11:35Z 2019-08-23 journal article Bellocchi, C.; Fernández-Ochoa, Á.; Montanelli, G.; Vigone, B.; Santaniello, A.; Quirantes-Piné, R.; Borrás-Linares, I.; Gerosa, M.; Artusi, C.; Gualtierotti, R.; Segura-Carrettero, A.; Alarcón-Riquelme, M.E.; Beretta, L. Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases. J. Clin. Med. 2019, 8, 1291. [doi:10.3390/jcm8091291] http://hdl.handle.net/10481/57644 10.3390/jcm8091291 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI