Transcriptome dynamics of the Myxococcus xanthus multicellular developmental program Muñoz Dorado, José Moraleda Muñoz, Aurelio Marcos Torres, Francisco Javier Contreras Moreno, Francisco Javier Pérez Torres, Juana The bacterium Myxococcus xanthus exhibits a complex multicellular life cycle. In the presence of nutrients, cells prey cooperatively. Upon starvation, they enter a developmental cycle wherein cells aggregate to produce macroscopic fruiting bodies filled with resistant myxospores. We used RNA-Seq technology to examine the transcriptome of the 96 hr developmental program. These data revealed that 1415 genes were sequentially expressed in 10 discrete modules, with expression peaking during aggregation, in the transition from aggregation to sporulation, or during sporulation. Analysis of genes expressed at each specific time point provided insights as to how starving cells obtain energy and precursors necessary for assembly of fruiting bodies and into developmental production of secondary metabolites. This study offers the first global view of developmental transcriptional profiles and provides important tools and resources for future studies. 2019-10-30T08:37:44Z 2019-10-30T08:37:44Z 2019-10-14 info:eu-repo/semantics/article Muñoz-Dorado, J., Moraleda-Muñoz, A., Marcos-Torres, F. J., Contreras-Moreno, F. J., Martin-Cuadrado, A. B., Schrader, J. M., ... & Pérez, J. (2019). Transcriptome dynamics of the Myxococcus xanthus multicellular developmental program. eLife, 8. http://hdl.handle.net/10481/57600 10.7554/eLife.50374 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España eLife Sciences Publications