<rdf:RDF xmlns:rdf="http://www.openarchives.org/OAI/2.0/rdf/" xmlns:ow="http://www.ontoweb.org/ontology/1#" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:ds="http://dspace.org/ds/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:doc="http://www.lyncode.com/xoai" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/rdf/ http://www.openarchives.org/OAI/2.0/rdf.xsd">
   <ow:Publication rdf:about="oai:digibug.ugr.es:10481/57496">
      <dc:title>Effects of Therapeutic Doses of Celecoxib on Several Physiological Parameters of Cultured Human Osteoblasts</dc:title>
      <dc:creator>Costela Ruiz, Víctor Javier</dc:creator>
      <dc:creator>Melguizo Rodríguez, Lucía Raquel</dc:creator>
      <dc:creator>Illescas Montes, Rebeca</dc:creator>
      <dc:creator>Ramos Torrecillas, Javier</dc:creator>
      <dc:creator>Manzano Moreno, Francisco Javier</dc:creator>
      <dc:creator>Ruiz Rodríguez, Concepción</dc:creator>
      <dc:creator>Luna Bertos, María Elvira De</dc:creator>
      <dc:subject>Celecoxib</dc:subject>
      <dc:subject>Human osteoblasts</dc:subject>
      <dc:subject>Osteoblast differentiation</dc:subject>
      <dc:subject>Bone</dc:subject>
      <dc:subject>COX-2 selective NSAIDs</dc:subject>
      <dc:description>Non-steroidal anti-inflammatory drugs (NSAIDs), including cyclooxygenase-2 (COX-2)-selective&#xd;
NSAIDs, are associated with adverse effects on bone tissue. These drugs are frequently the&#xd;
treatment of choice but are the least studied with respect to their repercussion on bone. The&#xd;
objective of this study was to determine the effects of celecoxib on cultured human osteoblasts.&#xd;
Human osteoblasts obtained by primary culture from bone samples were treated with celecoxib at&#xd;
doses of 0.75, 2, or 5μM for 24 h. The MTT technique was used to determine the effect on&#xd;
proliferation; flow cytometry to establish the effect on cell cycle, cell viability, and antigenic profile;&#xd;
and real-time polymerase chain reaction to measure the effect on gene expressions of the&#xd;
differentiation markers RUNX2, alkaline phosphatase (ALP), osteocalcin (OSC), and osterix (OSX).&#xd;
Therapeutic doses of celecoxib had no effect on osteoblast cell growth or antigen expression but&#xd;
had a negative impact on the gene expression of RUNX2 and OSC, although there was no significant&#xd;
change in the expression of ALP and OSX. Celecoxib at therapeutic doses has no apparent adverse&#xd;
effects on cultured human osteoblasts and only inhibits the expression of some differentiation&#xd;
markers. These characteristics may place this drug in a preferential position among NSAIDs used for&#xd;
analgesic and anti-inflammatory therapy during bone tissue repair.</dc:description>
      <dc:date>2019-10-24T10:44:20Z</dc:date>
      <dc:date>2019-10-24T10:44:20Z</dc:date>
      <dc:date>2019-09-20</dc:date>
      <dc:type>journal article</dc:type>
      <dc:identifier>Costela-Ruiz, V. J., Melguizo-Rodríguez, L., Illescas-Montes, R., Ramos-Torrecillas, J., Manzano-Moreno, F. J., Ruiz, C., &amp; De Luna-Bertos, E. (2019). Effects of Therapeutic Doses of Celecoxib on Several Physiological Parameters of Cultured Human Osteoblasts. Int J Med Sci, 16(11), 1466-1472.</dc:identifier>
      <dc:identifier>http://hdl.handle.net/10481/57496</dc:identifier>
      <dc:identifier>10.7150/ijms.37857</dc:identifier>
      <dc:language>eng</dc:language>
      <dc:rights>http://creativecommons.org/licenses/by/3.0/es/</dc:rights>
      <dc:rights>open access</dc:rights>
      <dc:rights>Atribución 3.0 España</dc:rights>
      <dc:publisher>Ivyspring International Publisher</dc:publisher>
   </ow:Publication>
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