Recovery of Oligomeric Proanthocyanidins and Other Phenolic Compounds with Established Bioactivity from Grape Seed By-Products Pasini, Federica Chinnici, Fabio Caboni, Maria Fiorenza Verardo, Vito Grape seeds Flavan-3-ols Oligomeric proanthocyanidins Phenolic compounds LH-20 Sephadex Grape seeds are a copious part of the grape pomace produced by wine and juice industry and they represent an interesting source of phenolic compounds. Proanthocyanidins (PAs) are the main class of grape seed phenols and are important dietary supplements for their well-known beneficial properties. In this study enriched extracts obtained from Chardonnay and Pignoletto grape seeds were characterized for their proanthocyanidins and other minor phenolic compounds content and composition. Seed PAs were fractionated using Sephadex LH-20, using different ethanol aqueous solutions as mobile phase and analysed by normal phase HPLC-FLD-ESI-MS. Monomers, oligomers up to dodecamers and polymers were recorded in all samples. For both cultivars, the extracts showed a high content in PAs. The determination of other phenolic compounds was carried out using a HPLC-QqQ-ESI-MS and Chardonnay samples reported a greater content compared to Pignoletto samples. Contrary to PAs fraction, extracts obtained with ethanol/water 50/50 (v/v) presented a significant higher phenolic content than the others. 2019-03-04T12:25:12Z 2019-03-04T12:25:12Z 2019-02-14 journal article Pasini, Federica; Chinnici, Fabio; Caboni, Maria Fiorenza; Verardo, Vito. Recovery of Oligomeric Proanthocyanidins and Other Phenolic Compounds with Established Bioactivity from Grape Seed By-Products. Molecules 2019, 24, 677. [http://hdl.handle.net/10481/54881] 1420-3049 http://hdl.handle.net/10481/54881 eng http://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España MDPI