Dental color measurement to predict DNA concentration in incinerated teeth for human identification Rubio, Leticia Sioli, Jose Manuel Gaitán, Maria Jesús Martín De Las Heras, Stella Abstract Teeth exposed to thermal stress can shed light on the identification of incinerated individuals and on the circumstances of the fire. Changes in the color of burned teeth can provide information on structural changes and the temperature of exposure. The objective of this study was to correlate color modifications with the concentration of human DNA in teeth burned at different temperatures. Spectrophotometry was used to measure the color of 40 teeth heated at temperatures of 100, 200, and 400ÊC for 60 min. DNA was extracted by phenol-chloroform extraction and quantified by real-time quantitative PCR using the Quantifier human DNA quantification kit. Preliminary results indicated an association of higher temperature with changes in colorimetric variables and a decrease in DNA concentrations. A significant positive correlation was found between luminosity values and DNA concentration (r = 0.4727, p = 0.0128) and between chromaticity a* values and DNA concentration (r = 0.4154, p = 0.0250). Spectrophotometry analysis of the color of burned teeth may predict the feasibility of extracting human DNA for identification purposes. 2019-01-24T08:06:31Z 2019-01-24T08:06:31Z 2018 info:eu-repo/semantics/article Rubio L, Sioli JM, GaitaÂn MJ, Martin-de- las-Heras S (2018) Dental color measurement to predict DNA concentration in incinerated teeth for human identification. PLoS ONE 13(4): e0196305.. [http://hdl.handle.net/10481/54623] 1932-6203 http://hdl.handle.net/10481/54623 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España David A Lightfoot, College of Agricultural Sciences, UNITED STATES