Development of polymorphic microsatellite markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae) García-López, María del Carmen Ben-Menni Schuler, Samira López Flores, Inmaculada Nieto-Lugilde, Marta Terrón Camero, Laura Carmen Suárez Santiago, Víctor Endangered fern Hymenophyllaceae Microsatellite markers Population genetics Vandenboschia speciosa Premise of the study: We characterize 10 microsatellite loci in the endangered fern Vandenboschia speciosa (Hymenophyllaceae), enabling studies on the genetic population structure of this Macaronesian-European species using DNA hypervariable markers. Methods and Results: Ten primer sets were developed and tested on 47 individuals in a total of two Iberian populations of V. speciosa. The primers amplified di- and hexanucelotide repeats. The number of alleles ranged from two to eight, and the expected heterozygosity ranged from 0.107 to 0.807 among the populations analyzed. Conclusions: The 10 microsatellite markers developed will be useful in characterizing the genetic diversity of V. speciosa and understanding its population structure (including the possible structure between sporophyte and gametophyte phases) and biogeographic history, and will provide important genetic data for the conservation of this species. 2018-01-30T13:23:11Z 2018-01-30T13:23:11Z 2015-11-04 info:eu-repo/semantics/article García-López, M.C.; et al. Development of polymorphic microsatellite markers for the Killarney Fern (Vandenboschia speciosa, Hymenophyllaceae). Applications in Plant Sciences, 3(11): 1500067 (2015). [http://hdl.handle.net/10481/49236] 2168-0450 http://hdl.handle.net/10481/49236 10.3732/apps.1500067 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Botanical Society of America; Wiley