A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study Toledano Pérez, Manuel Osorio Ruiz, Raquel Osorio Ruiz, María Estrella Cabello Malagón, Inmaculada Toledano Osorio, Manuel Sánchez Aguilera, Fátima Dentin Load cycling Confocal microscopy Zn Adhesive The aim of this study was to understand the effect of Zn-doping of adhesives and mechanical load cycling on the micromorphology of the resin-dentin interdiffusion zone (of sound and caries affected dentin). The investigation considered two different Zn-doped adhesive approaches and evaluated the interface using confocal laser scanning microscopy. Sound and carious dentin-resin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rhodamine B-labeled hybrid layer and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching and more extended in carious dentin. Zn-doping promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid layer porosity, and an increment of dentin mineralization. Load cycled augmented the sealing of the Zn-doped resin-dentin interfaces, as porosity and nanoleakage diminished, and even disappeared in caries-affected dentin substrata conditioned with EDTA. Sound and carious dentin specimens treated with the xylenol orange technique produced a clearly outlined fluorescence when resins were Zn-doped, due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when load cycling was applied on specimens treated with Zn-doped bonding components of self-etching adhesives. Micropermeability at the resin-dentin interface diminished after combining EDTA pretreatment, ZnCl2-doping and mechanical loading stimuli on restorations. It is clearly preferable to include the zinc compounds into the bonding constituents of the self-etching adhesives, instead of into the primer ingredients. The promoted new mineral segments contributed to reduce or avoid both porosity and nanoleakage from the load cycled Zn-doped resin dentin interface. 2017-06-09T06:22:50Z 2017-06-09T06:22:50Z 2017-05 info:eu-repo/semantics/preprint Toledano Pérez, M.; et al. A zinc chloride-doped adhesive facilitates sealing at the dentin interface: A confocal laser microscopy study. Journal of the Mechanical Behavior of Biomedical Materials, 74: 35-42 (2017). [http://hdl.handle.net/10481/46754] 1751-6161 http://hdl.handle.net/10481/46754 10.1016/j.jmbbm.2017.04.030 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Elsevier