A study of the role of KEA1 and KEA2 K+/H+ antiporters in chloroplast development and division in arabidopsis thaliana Aboukila, Ali Mohamed Ali Rodríguez Rosales, Pilar Universidad de Granada. Departamento de Fisiología Vegetal Consejo Superior de Investigaciones Científicas (CSIC). Estación Experimental del Zaidín Plantas Potasio Fotosíntesis Arabidopsis thaliana Levaduras (Botánica) Cloroplastos Genética Homeóstasis The general objective of this work was to study the physiological function of the Arabidopsis thaliana AtKEA1 and AtKEA2 proteins. For this purpose we have analyzed the phenotypes of single and double T-DNA knock-out mutants, determined the subcellular location of AtKEA2 and studied the function of AtKEA2 N-terminal domain by heterologous expression in yeast and by bioinformatics approaches. This general objective was developed through the following specific objectives: 1- Phenotypic analysis of single and double mutants. 2- Analysis of chloroplast number, morphology, ultrastructure. 3- Analysis of AtKEA2 subcellular localization and tissue specific expression. 4- Study of AtKEA2 interaction with chloroplast division proteins. 5- Bioinformatics and biochemical analysis of the N-terminal domain. 2017-03-01T11:35:54Z 2017-03-01T11:35:54Z 2017 2016-12-16 doctoral thesis Aboukila, A.M.A. A study of the role of KEA1 and KEA2 K+/H+ antiporters in chloroplast development and division in arabidopsis thaliana. Granada: Universidad de Granada, 2017. [http://hdl.handle.net/10481/45095] 9788491631200 http://hdl.handle.net/10481/45095 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Universidad de Granada