miRNA-146A regula la interacción entre células epiteliales intestinales, microbiota y estímulos inflamatorios Anzola Santander, Andrea Zarzuelo Zurita, Antonio Martínez-Agustín, Olga González Pérez, Raquel Universidad de Granada. Departamento de Farmacología Intestinos Microbiología Sistema inmunológico Bacterias Inflamación (Patología) Acido ribonucleico mensajero Células epiteliales We proposed 5 main objetives in this doctoral thesis: 1. To determine changes in miRNA expression in human intestinal epithelial cells, depending on the stage of cell differentiation and inflammation. 2. To study miR-146a expression in three animal models of colitis, that differ in the involvement of the intestinal mucosal barrier and, accordingly, in the level of contact with the intestinal microbiota. 3. To study the effect of bacterial antigens and cytokines on the expression of miR-146a using IEC lines. 4. To confirm the hypothesis that miR-146a may contribute to prevent overstimulation of the immune response. 5. To study the effect of bacterial antigens and cytokines on the expression of miR-146a using organoids from mouse small intestine. 2016-12-20T12:16:20Z 2016-12-20T12:16:20Z 2016 2016-07-08 doctoral thesis Anzola Santander, A. miRNA-146A regula la interacción entre células epiteliales intestinales, microbiota y estímulos inflamatorios. Granada: Universidad de Granada, 2016. [http://hdl.handle.net/10481/44082] 9788491259909 http://hdl.handle.net/10481/44082 spa http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Universidad de Granada