Polyaspartic acid enhances dentine remineralization bonded with a zinc-doped Portland-based resin cement Osorio Ruiz, Raquel Sauro, Salvatore Watson, Timothy F. Toledano Pérez, Manuel Bonding Dentine Remineralization Resin "This is the pre-peer reviewed version of the following article: "Osorio R, Sauro S, Watson TF, Toledano M. Polyaspartic acid enhances dentine remineralization bonded with a zinc-doped Portland-based resin cement. International Endodontic Journal", which has been published in final form at http://dx.doi.org/10.1111/iej.12518. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving." Aim. To ascertain if biomimetic phosphoproteins analogues (polyaspartic acid -PAS- and sodium trimetaphosphate -TMP-) improve bonding efficacy and dentine remineralization ability of a novel zinc-doped Portland-based resinous sealing cement. Methodology. Bonding procedures were performed on phosphoric acid etched dentine and several groups were established regarding biomimetic analogue application: 1) No application, 2) PAS-treated dentine and 3) dentine treated with a mixture of PAS and TMP. Raman spectroscopy and microtensile bond strength (MTBS) with fracture analysis by scanning electron microscopy were carried out. MTBS values were compared by ANOVA, Student-Newman-Keuls and Student t tests (P<0.05 and P<0.01 respectively). Results. MTBS values were not affected by the different bonding procedures, at 24 h testing. After 6 months, MTBS decreased in those groups in which analogues phosphoproteins’ were not applied (P<0.05). When Pas was applied, MTBS was maintained after 6 months (P>0.05). The novel material bonded without primers application induced bioactive crystal (calcium carbonate and Ettringite) precipitation onto the etched dentine and augmented the degree of crystallinity at the hybrid layer. Mineral to matrix ratio was increased at the hybrid layer of the PAS-treated specimens; this primer was also able to catalyze dentine remineralization, without an increase in crystallinity. Conclusions: PAS application onto demineralized dentine produced an inhibition or delay of the mineral phase crystallization, enhancing the remineralization potential of the Portland microfillers at the resin-dentine bonded interface. 2015-11-26T13:31:57Z 2015-11-26T13:31:57Z 2015-08 preprint Osorio, R.; Sauro, S.; Watson, T.F.; Toledano Pérez, M. Polyaspartic acid enhances dentine remineralization bonded with a zinc-doped Portland-based resin cement. International Endodontic Journal, 48: online (2015). [http://hdl.handle.net/10481/39011] 1365-2591 0143-2885 http://hdl.handle.net/10481/39011 10.1111/iej.12518 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License John Wiley and Sons