Generation and Characterization of Novel Magnetic Field-Responsive Biomaterials López López, Modesto Torcuato Scionti, Giuseppe Oliveira, Ana C. García López-Durán, Juan De Dios Muñoz Campos, Antonio Alaminos Mingorance, Miguel Rodríguez, Ismael Ángel Magnetic field-responsive Biomaterials Nanoparticles Mechanical properties Deformation Polymers Tissue mechanics Fibrin We report the preparation of novel magnetic field-responsive tissue substitutes based on biocompatible multi-domain magnetic particles dispersed in a fibrin–agarose biopolymer scaffold. We characterized our biomaterials with several experimental techniques. First we analyzed their microstructure and found that it was strongly affected by the presence of magnetic particles, especially when a magnetic field was applied at the start of polymer gelation. In these samples we observed parallel stripes consisting of closely packed fibers, separated by more isotropic net-like spaces. We then studied the viability of oral mucosa fibroblasts in the magnetic scaffolds and found no significant differences compared to positive control samples. Finally, we analyzed the magnetic and mechanical properties of the tissue substitutes. Differences in microstructural patterns of the tissue substitutes correlated with their macroscopic mechanical properties. We also found that the mechanical properties of our magnetic tissue substitutes could be reversibly tuned by noncontact magnetic forces. This unique advantage with respect to other biomaterials could be used to match the mechanical properties of the tissue substitutes to those of potential target tissues in tissue engineering applications. 2015-09-25T11:16:17Z 2015-09-25T11:16:17Z 2015-07-24 journal article López-López, M.T.; et al. Generation and Characterization of Novel Magnetic Field-Responsive Biomaterials. Plos One, 10(7): e0133878 (2015). [http://hdl.handle.net/10481/37665] 1932-6203 http://hdl.handle.net/10481/37665 10.1371/journal.pone.0133878 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Public Library of Science (PLOS)