Determination of bisphenol A and related aromatic compounds released from bis-GMA-based composites and sealants by high performance liquid chromatography Pulgar Encinas, Rosa María Olea Serrano, Fátima Novillo-Fertrell, Arancha Rivas, A. Pazos, Patricia Pedraza Muriel, Vicente Navajas, José M. Olea Serrano, Nicolás Bis-GMA Bisphenol A Bisphenol A dimethacrylate Dental composites and sealants 'Reproduced with permission from Environmental Health Perspectives' Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis-GMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytic methods. Concerns about the estrogenicity of bisphenol A (BPA) and other aromatic components leached from commercial products have been expressed. We studied biphenolic components eluted from seven composites and one sealant before and after in vitro polymerization using HPLC and gas chromatography/mass spectrometry and we investigated how pH modifications affect the leaching of these components. We found BPA (maximal amount 1.8 microg/mg dental material), its dimethacrylate derivative (Bis-DMA, 1.15 microg/mg), bisphenol A diglycidylether (6. 1 microg/mg), Bis-GMA (2.0 microg/mg), and ethoxylate and propoxylate of bisphenol A in media in which samples of different commercial products were maintained under controlled pH and temperature conditions. Our results confirm the leaching of estrogenic monomers into the environment by Bis-GMA-based composites and sealants in concentrations at which biologic effects have been demonstrated in in vivo experimental models. The main issue with implications for patient care and dentist responsibility is to further determine the clinical relevance of this estrogenic exposure. 2013-05-07T12:42:13Z 2013-05-07T12:42:13Z 2000 journal article Pulgar, R.; et al. Determination of bisphenol A and related aromatic compounds released from bis-GMA-based composites and sealants by high performance liquid chromatography. Environmental Health Perspectives, 108(1): 21-27 (2000). [http://hdl.handle.net/10481/24961] 0091-6765 1552-9924 PMCID: PMC1637852 http://hdl.handle.net/10481/24961 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ open access Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License National Institute of Environmental Health Sciences